RESUMEN
Urinary diversion after cystectomy has been a historical standard for the treatment of numerous benign and malignant diseases of the bladder. Since the first published description in the early 1900s, improvements in surgical technique and a better understanding of the metabolic sequelae postoperatively have greatly enhanced patient outcomes. Both continent and incontinent diversions are available to patients after cystectomy. In appropriately selected patients, orthotopic neobladder reconstruction can offer preservation of body image and continence, and continent cutaneous diversions represent a reasonable alternative. Conduit diversion, which remains the most commonly performed diversion technique, is ideal for patients who would benefit from a less morbid surgical procedure that negates the need for self-catheterization. This installment of the Core Curriculum in Nephrology outlines numerous aspects of urinary diversion, in which a multidisciplinary approach to postoperative management at the intersection of nephrology and urology is required to effectively optimize patient outcomes. This article includes a discussion of the various reconstructive options after cystectomy as well as a comprehensive review of frequently encountered short-term and long-term metabolic abnormalities associated with altered electrolyte and acid-base homeostasis.
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Cistectomía , Derivación Urinaria , Desequilibrio Ácido-Base/metabolismo , Desequilibrio Ácido-Base/terapia , Diarrea/metabolismo , Diarrea/terapia , Humanos , Síndromes de Malabsorción/metabolismo , Síndromes de Malabsorción/terapia , Nefrología , Cuidados Posoperatorios , Complicaciones Posoperatorias/metabolismo , Complicaciones Posoperatorias/terapia , Reservorios Urinarios Continentes , Urolitiasis/metabolismo , Urolitiasis/terapia , Urología , Desequilibrio Hidroelectrolítico/metabolismo , Desequilibrio Hidroelectrolítico/terapiaRESUMEN
BACKGROUND: We have previously investigated the fate of administered bicarbonate infused as a hypertonic solution in animals with each of the 4 chronic acid-base disorders. Those studies did not address the fate of sodium, the coadministered cation. METHODS: We examined baseline total body water (TBW), Na+ space, HCO3- space, and urinary sodium and bicarbonate excretion after acute hypertonic NaHCO3 infusion (1-N solution, 5 mmol/kg body weight) in dogs with each of the 4 chronic acid-base disorders. Observations were made at 30, 60, and 90 min postinfusion. Retained sodium that remains osmotically active distributes in an apparent space that approximates TBW. Na+ space that exceeds TBW uncovers nonosmotic sodium storage. RESULTS: Na+ space approximated TBW at all times in normal and hyperbicarbonatemic animals (metabolic alkalosis and respiratory acidosis), but exceeded TBW by ~30% in hypobicarbonatemic animals (metabolic acidosis and respiratory alkalosis). Such osmotic inactivation was detected at 30 min and remained stable. The pooled data revealed that Na+ space corrected for TBW was independent of the initial blood pH but correlated with initial extracellular bicarbonate concentration (y = -0.01x + 1.4, p= 0.002). The fate of administered sodium and bicarbonate (internal distribution and urinary excretion) was closely linked. CONCLUSIONS: This study demonstrates that hypobicarbonatemic animals have a Na+ space that exceeds TBW after an acute infusion of hypertonic NaHCO3 indicating osmotic inactivation of a fraction of retained sodium. In addition to an expanded Na+ space, these animals have a larger HCO3- space compared with hyperbicarbonatemic animals. Both phenomena appear to reflect the wider range of titration of nonbicarbonate buffers (Δ pH) occurring during NaHCO3- loading whenever initial [HCO3-]e is low. The data indicate that the fate of administered bicarbonate drives the internal distribution and the external disposal of sodium, the co-administered cation, and is responsible for the early, but non-progressive, osmotic inactivation of a fraction of the retained sodium.
Asunto(s)
Bicarbonato de Sodio/farmacocinética , Sodio/metabolismo , Desequilibrio Hidroelectrolítico/metabolismo , Animales , Cationes Monovalentes/sangre , Cationes Monovalentes/metabolismo , Cationes Monovalentes/orina , Modelos Animales de Enfermedad , Perros , Femenino , Humanos , Concentración de Iones de Hidrógeno , Soluciones Hipertónicas , Infusiones Intravenosas , Riñón , Eliminación Renal/fisiología , Sodio/sangre , Sodio/orina , Bicarbonato de Sodio/administración & dosificación , Distribución Tisular , Desequilibrio Hidroelectrolítico/sangre , Desequilibrio Hidroelectrolítico/tratamiento farmacológico , Desequilibrio Hidroelectrolítico/orinaRESUMEN
BACKGROUND: Overhydration (OH) is common in chronic kidney disease (CKD) and might be related to the excretion of urinary serine proteases. Progression of CKD is associated with proteinuria; however, the interrelations of urinary serine proteases, OH, and progression of CKD remain unclear. METHODS: In n = 179 patients with stable nondialysis-dependent CKD of all stages, OH was measured using bioimpedance spectroscopy (Body Composition Monitor; Fresenius), and urinary serine protease activity was determined using the peptide substrate S-2302. After a median follow-up of 5.9 (IQR: 3.9-6.5) years, progression to end-stage renal disease (ESRD) was analyzed retrospectively. RESULTS: OH correlated with baseline MDRD-eGFR, urinary albumin creatinine ratio (ACR), and urinary aprotinin-sensitive serine protease activity. Progression to ESRD occurred in n = 33 patients (19%) and correlated with OH and urinary serine protease activity as well as MDRD-eGFR and ACR. Patients were divided into 2 groups determined by cutoff values from receiver operating characteristics for MDRD-eGFR (32 mL/min/1.73 m2), ACR (43 mg/g creatinine), urinary serine protease activity (0.9 RU/g creatinine), and OH (1 L/1.73 m2). Across these cutoff values, Kaplan-Meier curves for renal survival showed significant separations of the groups. In Cox regression adjusted for MDRD-eGFR, ACR, P-NT-pro-BNP, systolic blood pressure, and diabetes mellitus, patients with OH >1 L/1.73 m2 had a 3.32 (95% CI: 1.26-8.76)-fold higher risk for progression to ESRD. CONCLUSIONS: Our results corroborate that OH detected by bioimpedance spectroscopy in CKD patients is an independent risk factor for progression to ESRD in addition to GFR and albuminuria. Urinary serine protease activity is associated with OH and progression of CKD and provides a possible underlying mechanism.
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Insuficiencia Renal Crónica/complicaciones , Desequilibrio Hidroelectrolítico/complicaciones , Agua/metabolismo , Anciano , Progresión de la Enfermedad , Femenino , Humanos , Masculino , Persona de Mediana Edad , Pronóstico , Insuficiencia Renal Crónica/diagnóstico , Insuficiencia Renal Crónica/metabolismo , Estudios Retrospectivos , Desequilibrio Hidroelectrolítico/diagnóstico , Desequilibrio Hidroelectrolítico/metabolismoRESUMEN
BACKGROUND: Gitelman syndrome is a rare salt-losing renal tubular disorder associated with mutation of SLC12A3 gene, which encodes the Na-Cl co-transporter (NCCT). Gitelman syndrome is characterized by hypokalemia, metabolic alkalosis, hypomagnesemia, hypocalciuria, and renin-angiotensin-aldosterone system (RAAS) activation. Different SLC12A3 variants may lead to phenotypic variability and severity. METHODS: In this study, we reported the clinical features and genetic analysis of a Chinese pedigree diagnosed with Gitelman syndrome. RESULTS: The proband exhibited hypokalaemia, hypomagnesemia, metabolic alkalosis, but hypercalciuria and kidney stone formation. The increased urinary calcium excretion made it confused to Bartter syndrome. The persistent renal potassium wasting resulted in renal tubular lesions, and might affect urinary calcium reabsorption and excretion. Genetic analysis revealed mutations of SLC12A3 gene with c.433C > T (p.Arg145Cys), c.1077C > G (p.Asn359Lys), and c.1666C > T (p.Pro556Ser). Potential alterations of structure and function of NCCT protein due to those genetic variations of SLC12A3 are predicted. Interestingly, one sibling of the proband carried the same mutant sites and exhibited similar clinical features with milder phenotypes of hypokalemia and hypomagnesemia, but hypocalciuria rather than hypercalciuria. Family members with at least one wild type copy of SLC12A3 had normal biochemistry. With administration of spironolactone, potassium chloride and magnesium supplement, the serum potassium and magnesium were maintained within normal ranges. CONCLUSIONS: In this study, we identified compound mutations of SLC12A3 associated with varieties of clinical features. Further efforts are needed to investigate the diversity in clinical manifestations of Gitelman syndrome and its correlation with specific SLC12A3 mutations.
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Síndrome de Gitelman/genética , Adulto , Anciano , Alcalosis/genética , Alcalosis/metabolismo , Síndrome de Bartter/metabolismo , China , Femenino , Genotipo , Síndrome de Gitelman/metabolismo , Humanos , Hipercalciuria/genética , Hipercalciuria/metabolismo , Hipopotasemia/genética , Hipopotasemia/metabolismo , Magnesio/sangre , Masculino , Persona de Mediana Edad , Mutación , Linaje , Fenotipo , Eliminación Renal , Miembro 3 de la Familia de Transportadores de Soluto 12/genética , Desequilibrio Hidroelectrolítico/genética , Desequilibrio Hidroelectrolítico/metabolismoRESUMEN
BACKGROUND: Increased oxidative stress in end-stage renal disease is regarded as one of the important mechanisms in the atherosclerosis and muscle wasting. However, studies examining the clinical significance of oxidative stress by direct measurement of these markers and its association with volume status and sarcopenia are limited. METHODS: A follow-up cross-sectional study was performed in stable hemodialysis (HD) patients and serum protein carbonyl levels were measured as a biomarker of oxidative stress. Additionally, multi-frequency body composition analysis, handgrip strength (HGS) and nutritional assessments were performed at baseline. RESULTS: Eighty-eight patients undergoing HD were included and 30 (34.1%) patients died during a mean follow-up of 5.2 years. The mean patient age was 60.6 ± 13.5 years, and the mean HD duration was 50.8 ± 41.3 months. In total, 16 patients (18.2%) were overhydrated, 49 (55.7%) had low HGS and 36 (40.9%) had low muscle mass. Serum protein carbonyl levels were associated with serum levels of albumin, prealbumin and transferrin, hydration status and low HGS. Overhydration (odds ratio [OR] 7.01, 95% CI 1.77-27.79, p = 0.006), prealbumin (OR 0.91, 95% CI 0.83-0.99, p = 0.030), subjective global assessment (OR 3.52, 95% CI 1.08-11.46, p = 0.037) and sarcopenia (OR 3.41, 95% CI 1.02-11.32, p = 0.046) were significantly related to increased serum protein carbonyl levels. Multivariate analysis showed that the serum levels of protein carbonyl (Hazard ratio [HR] 2.37, 95% CI 1.02-5.55, p = 0.036), albumin (HR 0.17, 95% CI 0.06-0.46, p = 0.003), prealbumin (HR 0.86, 95% CI 0.80-0.92, p = 0.001), overhydration (HR 2.31, 95% CI 1.26-8.71, p = 0.015) and sarcopenia (HR 2.72, 95% CI 1.11-6.63, p = 0.028) were independent determinants of all-cause mortality. CONCLUSIONS: Serum protein carbonyl was significantly associated with overhydration, nutritional status and sarcopenia, and could be a new predictor of mortality in patients undergoing HD.
Asunto(s)
Fuerza de la Mano , Fallo Renal Crónico/metabolismo , Mortalidad , Estrés Oxidativo , Carbonilación Proteica , Sarcopenia/metabolismo , Albúmina Sérica/metabolismo , Transferrina/metabolismo , Desequilibrio Hidroelectrolítico/metabolismo , Anciano , Composición Corporal , Estudios Transversales , Femenino , Estudios de Seguimiento , Humanos , Fallo Renal Crónico/complicaciones , Fallo Renal Crónico/terapia , Masculino , Persona de Mediana Edad , Estado Nutricional , Oportunidad Relativa , Prealbúmina/metabolismo , Modelos de Riesgos Proporcionales , Diálisis Renal , Sarcopenia/complicaciones , Sarcopenia/fisiopatología , Desequilibrio Hidroelectrolítico/complicacionesRESUMEN
BACKGROUND: Renal loss of potassium (K+) and magnesium (Mg2+) in salt losing tubulopathies (SLT) leads to significantly reduced Quality of Life (QoL) and higher risks of cardiac arrhythmia. The normalization of K+ is currently the most widely accepted treatment target, however in even excellently designed RCTs the increase of K+ was only mild and rarely normalized. These findings question the role of K+ as the ideal marker of potassium homeostasis in SLT. Aim of this hypothesis-generating study was to define surrogate endpoints for future treatment trials in SLT in terms of their usefulness to determine QoL and important clinical outcomes. METHODS: Within this prospective cross-sectional study including 11 patients with SLTs we assessed the biochemical, clinical and cardiological parameters and their relationship with QoL (RAND SF-36). The primary hypothesis was that QoL would be more dependent of higher aldosterone concentration, assessed by the transtubular-potassium-gradient (TTKG). Correlations were evaluated using Pearson's correlation coefficient. RESULTS: Included patients were mainly female (82%, mean age 34 ± 12 years). Serum K+ and Mg2+ was 3.3 ± 0.6 mmol/l and 0.7 ± 0.1 mmol/l (mean ± SD). TTKG was 9.5/3.4-20.2 (median/range). While dimensions of mental health mostly correlated with serum Mg2+ (r = 0.68, p = 0.04) and K+ (r = 0.55, p = 0.08), better physical health was associated with lower aldosterone levels (r = -0.61, p = 0.06). TTKG was neither associated with aldosterone levels nor with QoL parameters. No relevant abnormalities were observed in neither 24 h-ECG nor echocardiography. CONCLUSIONS: Hyperaldosteronism, K+ and Mg2+ were the most important parameters of QoL. TTKG was no suitable marker for hyperaldosteronism or QoL. Future confirmatory studies in SLT should assess QoL as well as aldosterone, K+ and Mg2+.
Asunto(s)
Síndrome de Bartter/fisiopatología , Síndrome de Gitelman/fisiopatología , Hiperaldosteronismo/fisiopatología , Hipopotasemia/fisiopatología , Magnesio/metabolismo , Calidad de Vida , Adulto , Aldosterona/metabolismo , Síndrome de Bartter/metabolismo , Síndrome de Bartter/psicología , Femenino , Síndrome de Gitelman/metabolismo , Síndrome de Gitelman/psicología , Homeostasis , Humanos , Hiperaldosteronismo/metabolismo , Hiperaldosteronismo/psicología , Hipopotasemia/metabolismo , Hipopotasemia/psicología , Masculino , Persona de Mediana Edad , Potasio/metabolismo , Estudios Prospectivos , Desequilibrio Hidroelectrolítico/metabolismo , Desequilibrio Hidroelectrolítico/fisiopatología , Desequilibrio Hidroelectrolítico/psicología , Adulto JovenRESUMEN
BACKGROUND: Acute kidney injury (AKI) often occurs in critically ill patients. AKI is associated with mortality and morbidity. Interventions focusing on the reduction of AKI are suggested by the Kidney Disease: Improving Global Outcomes guideline. We hypothesized that these educational interventions would improve outcome in patients admitted to the Intensive Care Unit (ICU). METHODS: This was a pragmatic single-centre prospective observational before-after study design in an ICU in a tertiary referral hospital. All consecutive patients admitted to the ICU irrespective their illness were included. A 'Save the Kidney' (STK) bundle was encouraged via an educational intervention targeting health care providers. The educational STK bundle consisted of optimizing the fluid balance (based on urine output, serum lactate levels and/or central venous oxygen saturation), discontinuation of diuretics, maintaining a mean arterial pressure of at least 65 mmHg with the potential use of vasopressors and critical evaluation of the indication and dose of nephrotoxic drugs. The primary outcome was the composite of mortality, renal replacement therapy (RRT), and progression of AKI. Secondary outcomes were the components of the composite outcome the severity of AKI, ICU length of stay and in-hospital mortality. MAIN RESULTS: The primary outcome occurred in 451 patients (33%) in the STK group versus 375 patients (29%) in the usual care group, relative risk (RR) 1.16, 95% confidence interval (CI) 1.03-1.3, p < 0.001. Secondary outcomes were, ICU mortality in 6.8% versus 5.6%, (RR 1.22, 95% CI 0.90-1.64, p = 0.068), RRT in 1.6% versus 3.6% (RR 0.46, 95% CI 0.28-0.76, p = 0.002), and AKI progression in 28% versus 24% (RR 1.18, 95% CI 1.04-1.35, p = 0.001). CONCLUSIONS: Providing education to uniformly apply an AKI care bundle, without measurement of the implementation in a non-selected ICU population, targeted at prevention of AKI progression was not beneficial.
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Lesión Renal Aguda/terapia , Personal de Salud/educación , Mortalidad Hospitalaria , Paquetes de Atención al Paciente/métodos , Lesión Renal Aguda/metabolismo , Adulto , Anciano , Presión Arterial , Enfermedad Crítica , Deprescripciones , Progresión de la Enfermedad , Diuréticos/uso terapéutico , Femenino , Fluidoterapia/métodos , Humanos , Unidades de Cuidados Intensivos , Análisis de Series de Tiempo Interrumpido , Ácido Láctico/sangre , Tiempo de Internación/estadística & datos numéricos , Masculino , Persona de Mediana Edad , Mortalidad , Oxígeno/sangre , Estudios Prospectivos , Terapia de Reemplazo Renal/estadística & datos numéricos , Centros de Atención Terciaria , Vasoconstrictores/uso terapéutico , Desequilibrio Hidroelectrolítico/metabolismo , Desequilibrio Hidroelectrolítico/terapiaRESUMEN
Afferent arteriole (Af-Art) diameter regulates pressure and flow into the glomerulus, which are the main determinants of the glomerular filtration rate. Thus, Af-Art resistance is crucial for Na+ filtration. Af-Arts play a role as integrative centers, where systemic and local systems interact to determine the final degree of resistance. The tubule of a single nephron contacts an Af-Art of the same nephron at two locations: in the transition of the thick ascending limb to the distal tubule (macula densa) and again in the connecting tubule. These two sites are the anatomic basis of two intrinsic feedback mechanisms: tubule-glomerular feedback and connecting tubule-glomerular feedback. The cross communications between the tubules and Af-Arts integrate tubular Na+ and water processing with the hemodynamic conditions of the kidneys. Tubule-glomerular feedback provides negative feedback that tends to avoid salt loss, and connecting tubule-glomerular feedback provides positive feedback that favors salt excretion by modulating tubule-glomerular feedback (resetting it) and increasing glomerular filtration rate. These feedback mechanisms are also exposed to systemic modulators (hormones and the nervous system); however, they can work in isolated kidneys or nephrons. The exaggerated activation or absence of any of these mechanisms may lead to disequilibrium in salt and water homeostasis, especially in extreme conditions (e.g., high-salt diet/low-salt diet) and may be part of the pathogenesis of some diseases. In this review, we focus on molecular signaling, feedback interactions, and the physiological roles of these two feedback mechanisms.
Asunto(s)
Canales Epiteliales de Sodio/metabolismo , Tasa de Filtración Glomerular , Glomérulos Renales/irrigación sanguínea , Túbulos Renales/metabolismo , Circulación Renal , Sodio/metabolismo , Equilibrio Hidroelectrolítico , Animales , Retroalimentación Fisiológica , Hemodinámica , Humanos , Túbulos Renales/fisiopatología , Desequilibrio Hidroelectrolítico/metabolismo , Desequilibrio Hidroelectrolítico/fisiopatologíaRESUMEN
Balb/CJ mice are more sensitive to treatment with angiotensin II (ANG II) and high-salt diet compared with C57BL/6J mice. Together with higher mortality, they develop edema, signs of heart failure, and acute kidney injury. The aim of the present study was to identify differences in renal gene regulation that may affect kidney function and fluid balance, which could contribute to decompensation in Balb/CJ mice after ANG II + salt treatment. Male Balb/CJ and C57BL/6J mice were divided into the following five different treatment groups: control, ANG II, salt, ANG II + salt, and ANG II + salt + N-acetylcysteine. Gene expression microarrays were used to explore differential gene expression after treatment and between the strains. Published data from the Mouse Genome Database were used to identify the associated genomic differences. The glomerular filtration rate (GFR) was measured using inulin clearance, and fluid balance was measured using metabolic cages. Gene ontology enrichment analysis of gene expression microarrays identified glutathione transferase (antioxidant system) as highly enriched among differentially expressed genes. Balb/CJ mice had similar GFR compared with C57BL/6J mice but excreted less Na+ and water, although net fluid and electrolyte balance did not differ, suggesting that Balb/CJ mice may be inherently more prone to decompensation. Interestingly, C57BL/6J mice had higher urinary oxidative stress despite their relative protection from decompensation. In addition, treatment with the antioxidant N-acetylcysteine decreased oxidative stress in C57BL/6J mice, reduced urine excretion, and increased mortality. Balb/CJ mice are more sensitive than C57BL/6J to ANG II + salt, in part mediated by lower oxidative stress, which favors fluid and Na+ retention.
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Angiotensina II , Tasa de Filtración Glomerular , Riñón/fisiopatología , Estrés Oxidativo , Cloruro de Sodio Dietético , Equilibrio Hidroelectrolítico , Desequilibrio Hidroelectrolítico/fisiopatología , Animales , Presión Sanguínea , Modelos Animales de Enfermedad , Femenino , Regulación de la Expresión Génica , Tasa de Filtración Glomerular/genética , Riñón/metabolismo , Masculino , Ratones Endogámicos BALB C , Ratones Endogámicos C57BL , Embarazo , Factores Sexuales , Especificidad de la Especie , Equilibrio Hidroelectrolítico/genética , Desequilibrio Hidroelectrolítico/etiología , Desequilibrio Hidroelectrolítico/genética , Desequilibrio Hidroelectrolítico/metabolismoRESUMEN
Diabetic kidney disease (DKD) is one of the leading pathological causes of decreased renal function and progression to end-stage kidney failure. To explore and characterize age-related changes in DKD and associated glomerular damage, we used a rat model of type 2 diabetic nephropathy (T2DN) at 12 wk and older than 48 wk. We compared their disease progression with control nondiabetic Wistar and diabetic Goto-Kakizaki (GK) rats. During the early stages of DKD, T2DN and GK animals revealed significant increases in blood glucose and kidney-to-body weight ratio. Both diabetic groups had significantly altered renin-angiotensin-aldosterone system function. Thereafter, during the later stages of disease progression, T2DN rats demonstrated a remarkable increase in renal damage compared with GK and Wistar rats, as indicated by renal hypertrophy, polyuria accompanied by a decrease in urine osmolarity, high cholesterol, a significant prevalence of medullary protein casts, and severe forms of glomerular injury. Urinary nephrin shedding indicated loss of the glomerular slit diaphragm, which also correlates with the dramatic elevation in albuminuria and loss of podocin staining in aged T2DN rats. Furthermore, we used scanning ion microscopy topographical analyses to detect and quantify the pathological remodeling in podocyte foot projections of isolated glomeruli from T2DN animals. In summary, T2DN rats developed renal and physiological abnormalities similar to clinical observations in human patients with DKD, including progressive glomerular damage and a significant decrease in renin-angiotensin-aldosterone system plasma levels, indicating these rats are an excellent model for studying the progression of renal damage in type 2 DKD.
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Diabetes Mellitus Tipo 2/patología , Nefropatías Diabéticas/patología , Envejecimiento , Albuminuria/etiología , Albuminuria/prevención & control , Animales , Glucemia/metabolismo , Progresión de la Enfermedad , Hipertrofia , Glomérulos Renales/patología , Masculino , Proteínas de la Membrana/orina , Tamaño de los Órganos , Poliuria/etiología , Poliuria/patología , Ratas , Ratas Wistar , Sistema Renina-Angiotensina , Desequilibrio Hidroelectrolítico/etiología , Desequilibrio Hidroelectrolítico/metabolismoRESUMEN
The (pro)renin receptor (PRR) is a new component of the renin-angiotensin-aldosterone system (RAAS) and regulates renin activity. The objective of the present study was to test potential roles of the renal PRR and intrarenal RAAS in the physiological status of late pregnancy. Late pregnant Sprague-Dawley rats were studied 19-21 days after sperm was observed in vaginal smears. Experiments were performed using age-matched virgin rats and late pregnant rats treated with the specific PRR inhibitor PRO20 (700 µg·kg-1·day-1 sc for 14 days, 3 times/day for every 8 h) or vehicle. The indices of RAAS, including PRR, renin, angiotensin II, and aldosterone levels, were examined by immunoblotting, qRT-PCR, or ELISA. Further analyses of renal epithelial sodium channel (ENaC) expression, sodium-water retention, and plasma volume were performed. We first present evidence for the activation of intrarenal RAAS in late pregnant rats, including increases in urinary renin activity, active and total renin content, and prorenin content, angiotensin II and aldosterone excretion, in parallel with increased renal PRR expression and urinary soluble PRR excretion. Functional evidence demonstrated that PRR antagonism with PRO20 effectively suppressed the indices of intrarenal RAAS in late pregnant rats. In addition, our results revealed that renal α-ENaC expression, sodium-water retention, and plasma volume were elevated during late pregnancy, which were all attenuated by PRO20. In summary, the present study examined the renal mechanism of sodium-water retention and plasma volume expansion in late pregnant rats and identified a novel role of PRR in regulation of intrarenal RAAS and α-ENaC and thus sodium and fluid retention associated with pregnancy.
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Canales Epiteliales de Sodio/metabolismo , Riñón/metabolismo , Receptores de Superficie Celular/metabolismo , Sistema Renina-Angiotensina/fisiología , Desequilibrio Hidroelectrolítico/metabolismo , Aldosterona/metabolismo , Angiotensina II/metabolismo , Animales , Presión Sanguínea/efectos de los fármacos , Canales Epiteliales de Sodio/genética , Femenino , Riñón/efectos de los fármacos , Fragmentos de Péptidos/farmacología , Embarazo , Ratas , Ratas Sprague-Dawley , Receptores de Superficie Celular/genética , Renina/farmacología , Sistema Renina-Angiotensina/efectos de los fármacos , Sodio/metabolismo , Agua/metabolismo , Receptor de ProreninaRESUMEN
The genetic background of a mouse strain determines its susceptibility to disease. C57BL/6J and Balb/CJ are two widely used inbred mouse strains that we found react dramatically differently to angiotensin II and high-salt diet (ANG II + Salt). Balb/CJ show increased mortality associated with anuria and edema formation while C57BL/6J develop arterial hypertension but do not decompensate and die. Clinical symptoms of heart failure in Balb/CJ mice gave the hypothesis that ANG II + Salt impairs cardiac function and induces cardiac remodeling in male Balb/CJ but not in male C57BL/6J mice. To test this hypothesis, we measured cardiac function using echocardiography before treatment and every day for 7 days during treatment with ANG II + Salt. Interestingly, pulsed wave Doppler of pulmonary artery flow indicated increased pulmonary vascular resistance and right ventricle systolic pressure in Balb/CJ mice, already 24 h after ANG II + Salt treatment was started. In addition, Balb/CJ mice showed abnormal diastolic filling indicated by reduced early and late filling and increased isovolumic relaxation time. Furthermore, Balb/CJ exhibited lower cardiac output compared with C57BL/6J even though they retained more sodium and water, as assessed using metabolic cages. Left posterior wall thickness increased during ANG II + Salt treatment but did not differ between the strains. In conclusion, ANG II + Salt treatment causes early restriction of pulmonary flow and reduced left ventricular filling and cardiac output in Balb/CJ, which results in fluid retention and peripheral edema. This makes Balb/CJ a potential model to study the adaptive capacity of the heart for identifying new disease mechanisms and drug targets.
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Angiotensina II/metabolismo , Síndrome Cardiorrenal/fisiopatología , Dieta , Hipertensión/fisiopatología , Animales , Presión Sanguínea/fisiología , Síndrome Cardiorrenal/complicaciones , Insuficiencia Cardíaca/fisiopatología , Hipertensión/complicaciones , Hipertensión Pulmonar/complicaciones , Masculino , Ratones Endogámicos BALB C , Miocardio/metabolismo , Cloruro de Sodio Dietético/metabolismo , Cloruro de Sodio Dietético/farmacología , Factores de Tiempo , Desequilibrio Hidroelectrolítico/tratamiento farmacológico , Desequilibrio Hidroelectrolítico/metabolismoRESUMEN
PURPOSE OF REVIEW: An understanding of fluid and electrolyte losses from diarrhea and mechanisms of solute cotransport led to development of oral rehydration solution (ORS), representing a watershed in efforts to reduce diarrheal disease morbidity and mortality. This report reviews the scientific rationale and modifications of ORS and barriers to universal application. RECENT FINDINGS: Solutions with osmolality and electrolyte composition different from original ORS for routine and unique pathophysiology such as in malnutrition have met with varying success. Following the conceptual rationale of sodium-glucose cotransportation to facilitate water absorption, other cotransporters and formulations have been explored with the aim to improve ORS efficacy and acceptance. ORS remains the anchor of acute watery diarrhea and dehydration management worldwide. Despite development of different formulations, the current standard solution is the mainstay of treatment for nearly all situations. Efforts to improve oral hydration solution and to increase acceptance and usage are ongoing.
Asunto(s)
Diarrea/terapia , Fluidoterapia/métodos , Soluciones para Rehidratación/farmacología , Soluciones para Rehidratación/uso terapéutico , Transportador 1 de Sodio-Glucosa/metabolismo , Desequilibrio Hidroelectrolítico/terapia , Administración Oral , Diarrea/complicaciones , Diarrea/metabolismo , Diarrea/fisiopatología , Humanos , Desequilibrio Hidroelectrolítico/etiología , Desequilibrio Hidroelectrolítico/metabolismo , Desequilibrio Hidroelectrolítico/fisiopatologíaRESUMEN
BACKGROUND: Diuresis has been observed within a week following renal transplantation, suggesting that the procedure causes acute disturbance of renal water homeostasis. Aquaporin (AQP) 1 and AQP2, important proteins for renal water reabsorption, have been identified in urinary extracellular vesicles (uEV-AQP1 and -AQP2), and experimental studies have shown that the presence of uEV-AQP1 and -AQP2 may be an indicator of their levels of expression in the kidney. However, the release patterns of uEV-AQP1 and -AQP2 during the acute phase following renal transplantation are largely unknown. METHODS: In this study, we examined the release of uEV-AQP1 and -AQP2 in recipients until 6 days (day 6) after renal transplantation. At Miyazaki prefectural Miyazaki Hospital, Japan, uEVs were obtained from 7 recipients, all of whom had received renal allografts from living donors. uEVs were isolated by differential centrifugation. RESULTS: Immunoblotting analysis showed that the release of uEV-AQP2 was significantly decreased on day 1 in comparison with a control sample (from 3 healthy volunteers), accompanied by high urine output and low urine osmolality. Thereafter, the level increased gradually to the control level by day 6. The release pattern of uEV-AQP1 was similar to that of uEV-AQP2, but the levels did not reach statistical significance in comparison with the control level at any of the time points examined. Evaluation of the relationship between urinary osmolality and uEV-AQPs revealed a significant correlation for uEV-AQP2, but not for uEV-AQP1. CONCLUSION: These results indicate that acute diuresis after renal transplantation might be due to a decrease in the renal expression of AQP2, whose level can be estimated from the amount released in uEVs.
Asunto(s)
Acuaporina 1/metabolismo , Acuaporina 2/metabolismo , Vesículas Extracelulares/metabolismo , Trasplante de Riñón , Complicaciones Posoperatorias , Reabsorción Renal/fisiología , Adulto , Femenino , Humanos , Fallo Renal Crónico/cirugía , Trasplante de Riñón/efectos adversos , Trasplante de Riñón/métodos , Masculino , Persona de Mediana Edad , Cuidados Posoperatorios/métodos , Complicaciones Posoperatorias/diagnóstico , Complicaciones Posoperatorias/etiología , Complicaciones Posoperatorias/metabolismo , Urinálisis/métodos , Desequilibrio Hidroelectrolítico/diagnóstico , Desequilibrio Hidroelectrolítico/etiología , Desequilibrio Hidroelectrolítico/metabolismoRESUMEN
Sodium is the most important osmotically effective cation in the extracellular space and very important for the water balance of the organism. Disturbances in the sodium homeostasis are therefore usually closely associated with disturbances in the water balance. The most important organs involved in the regulation of the sodium homeostasis are the kidneys and the brain. Disturbances of sodium homeostasis are common electrolyte disturbances, which can cause serious complications, as well as their improper therapy. The aim of this article is to inform about the etiology of disturbances of sodium homeostasis and to present important therapeutic principles.The TUR syndrome is a complication that can occur in the context of transurethral resection of the prostate which can lead to the inundation of larger amounts of hypotonic, electrolyte-free rinsing solution into the circulation and severe hyponatremia. Central pontine myelinolysis is a dangerous complication of a too fast compensation of hyponatremia. Exercise-associated hyponatremia (EHA) is a severe complication due to an inappropriately high intake of hypotonic fluids (mineral water, "isotonic sports drinks") during endurance sports. Due to the increasing popularity of endurance sports competitions (marathon, triathlon), an increasing incidence of this potentially life-threatening electrolyte disorder is to be expected.
Asunto(s)
Sodio/metabolismo , Desequilibrio Hidroelectrolítico/metabolismo , Homeostasis , Humanos , Hipernatremia/metabolismo , Hipernatremia/terapia , Hiponatremia/metabolismo , Hiponatremia/terapia , Periodo Perioperatorio , Desequilibrio Hidroelectrolítico/terapiaRESUMEN
Potassium is a key cation in the human organism and largely responsible for the resting membrane potential of excitable cells. 98% of the potassium pool resides within the cells and only 2% in the extracellular space. Thus, blood sample measurements of potassium do not necessarily reflect the total potassium state. The extracellular potassium concentration however is tightly regulated, either through potassium elimination via the kidney or shift between the intra- or extracellular compartment. Changes in the blood serum potassium concentration are defined as hypokalemia (< 3,5 mmol/l) or hyperkalemia (> 5,0 mmol/l). Hypokalemia is caused by a low-potassium diet, gastrointestinal losses or polyuric renal failure. Hyperkalemia can follow excessive intake, severe tissue damage or oliguric or anuric renal failure. Acute alteration of the extracellular potassium concentration should spark immediate action by the perioperative physician. In particular, hyperkalemic states require immediate attention and therapeutic interventions. In this review, we give an overview of the pathophysiology of potassium changes and provide a practical approach to their management.
Asunto(s)
Potasio/metabolismo , Desequilibrio Hidroelectrolítico/terapia , Homeostasis , Humanos , Hiperpotasemia/metabolismo , Hiperpotasemia/terapia , Hipopotasemia/metabolismo , Hipopotasemia/terapia , Potasio/fisiología , Desequilibrio Hidroelectrolítico/metabolismoRESUMEN
Disturbances of calcium homeostasis are common. Their pathophysiological causes are very heterogeneous and clinical symptoms are often non-specific. Therefore, an exact diagnosis is indispensable and a rapid compensation of the calcium homeostasis essential. This article discusses the pathophysiological, diagnostic and therapeutic principles of hypocalcemia and hypercalcemia and their implications for anaesthesia and intensive care.
Asunto(s)
Calcio/metabolismo , Desequilibrio Hidroelectrolítico/metabolismo , Homeostasis , Humanos , Hipercalcemia/metabolismo , Hipercalcemia/terapia , Hipocalcemia/metabolismo , Hipocalcemia/terapia , Periodo Perioperatorio , Desequilibrio Hidroelectrolítico/terapiaRESUMEN
Peptide receptor radionuclide therapy (PRRT) is a molecular-targeted therapy in which a somatostatin analogue (a small peptide) is coupled with a radioligand so that the radiation dose is selectively administered to somatostatin receptor-expressing metastasized neuroendocrine tumors, particularly gastroenteropancreatic. Reported toxicities include myelotoxicity and nephrotoxicity, the latter manifesting as decreased kidney function, often developing months to years after treatment completion. We present a case of PRRT-induced kidney toxicity manifesting as a severe Gitelman-like tubulopathy with preserved kidney function. Because profound hypokalemia and hypocalcemia can lead to life-threatening arrhythmias, we highlight the necessity for careful monitoring of serum and urine electrolytes in patients receiving PRRT.
Asunto(s)
Síndrome de Gitelman/inducido químicamente , Neoplasias del Íleon/radioterapia , Tumores Neuroendocrinos/radioterapia , Octreótido/análogos & derivados , Compuestos Organometálicos/efectos adversos , Desequilibrio Hidroelectrolítico/inducido químicamente , Acidosis/inducido químicamente , Acidosis/metabolismo , Acidosis/terapia , Anciano , Calcitriol/uso terapéutico , Carbonato de Calcio/uso terapéutico , Quimioradioterapia Adyuvante , Procedimientos Quirúrgicos del Sistema Digestivo , Fluidoterapia , Síndrome de Gitelman/metabolismo , Síndrome de Gitelman/terapia , Humanos , Hipocalcemia/inducido químicamente , Hipocalcemia/metabolismo , Hipocalcemia/terapia , Hipopotasemia/inducido químicamente , Hipopotasemia/metabolismo , Hipopotasemia/terapia , Sulfato de Magnesio/uso terapéutico , Masculino , Octreótido/efectos adversos , Vitaminas/uso terapéutico , Desequilibrio Hidroelectrolítico/metabolismo , Desequilibrio Hidroelectrolítico/terapiaRESUMEN
Nitric oxide (NO), carbon monoxide (CO), and hydrogen sulfide (H2S) are gaseous molecules produced by the brain. Within the hypothalamus, gaseous molecules have been highlighted as autocrine and paracrine factors regulating endocrine function. Therefore, in the present review, we briefly discuss the main findings linking NO, CO, and H2S to the control of body fluid homeostasis at the hypothalamic level, with particular emphasis on the regulation of neurohypophyseal system output.
Asunto(s)
Monóxido de Carbono/metabolismo , Sulfuro de Hidrógeno/metabolismo , Sistema Hipotálamo-Hipofisario/metabolismo , Óxido Nítrico/metabolismo , Transducción de Señal , Equilibrio Hidroelectrolítico , Desequilibrio Hidroelectrolítico/metabolismo , Animales , Comunicación Autocrina , Gases , Humanos , Sistema Hipotálamo-Hipofisario/fisiopatología , Comunicación Paracrina , Desequilibrio Hidroelectrolítico/fisiopatologíaRESUMEN
PURPOSE OF REVIEW: Identification of the mechanisms of magnesium absorption and reabsorption has markedly enhanced our understanding of the causes of hypomagnesemia. RECENT FINDINGS: New gastrointestinal and renal causes of hypomagnesemia have been recently documented. SUMMARY: The recognition of new mechanisms and causes of magnesium absorption and reabsorption should enhance the ability to monitor patients at risk for hypomagnesemia and improve our ability to mitigate the serious symptoms associated with this disorder.