RESUMEN
BACKGROUND: Many variations in oocyte and embryo grading make inter-laboratory comparisons extremely difficult. This paper reports the proceedings of an international consensus meeting on oocyte and embryo morphology assessment. METHODS: Background presentations about current practice were given. RESULTS: The expert panel developed a set of consensus points to define the minimum criteria for oocyte and embryo morphology assessment. CONCLUSIONS: It is expected that the definition of common terminology and standardization of laboratory practice related to embryo morphology assessment will result in more effective comparisons of treatment outcomes. This document is intended to be referenced as a global consensus to allow standardized reporting of the minimum data set required for the accurate description of embryo development.
Asunto(s)
Embrión de Mamíferos/citología , Desarrollo Embrionario/fisiología , Recuperación del Oocito/normas , Oocitos/citología , Técnicas Reproductivas Asistidas/normas , Blastómeros/citología , Aberraciones Cromosómicas , Fase de Segmentación del Huevo/clasificación , Humanos , Oocitos/clasificación , Terminología como Asunto , Resultado del TratamientoRESUMEN
This paper reports the proceedings of an international consensus meeting on oocyte and embryo morphology assessment. Following background presentations about current practice, the expert panel developed a set of consensus points to define the minimum criteria for oocyte and embryo morphology assessment. It is expected that the definition of common terminology and standardization of laboratory practice related to embryo morphology assessment will result in more effective comparisons of treatment outcomes. This document is intended to be referenced as a global consensus to allow standardized reporting of the minimum dataset required for the accurate description of embryo development. This paper reports the proceedings and outcomes of an international consensus meeting on human oocyte and embryo morphology assessment. An expert panel developed a series of consensus points to define the minimum criteria for such assessments. The definition of common terminology, and standardization of laboratory practices related to these morphological assessments, will permit more effective comparisons of treatment outcomes around the world. This report is intended to be referenced as a global consensus to allow standardized reporting of the minimum descriptive criteria required for routine clinical evaluations of human embryo development in vitro.
Asunto(s)
Embrión de Mamíferos/citología , Desarrollo Embrionario/fisiología , Recuperación del Oocito/normas , Oocitos/citología , Técnicas Reproductivas Asistidas/normas , Blastómeros/citología , Aberraciones Cromosómicas , Fase de Segmentación del Huevo/clasificación , Humanos , Oocitos/clasificación , Terminología como Asunto , Resultado del TratamientoRESUMEN
Oocytes with three or more pronuclei and one or two polar bodies arising after intracytoplasmic sperm injection were analyzed cytogenetically, and the data were used to develop a general model explaining the possible origin of the abnormal pronuclear stages. The mechanisms, either separately or in variable combinations, that induce the appearance of additional pronuclei are: 1) nonextrusion of the second polar body; 2) incomplete chromatid segregation into the extruded second polar body; and 3) dispersal of the oocyte chromatids in the presence of the second polar body formation.
Asunto(s)
Fase de Segmentación del Huevo , Formación de Concepto , Análisis Citogenético/métodos , Oocitos , Inyecciones de Esperma Intracitoplasmáticas , Aneuploidia , Núcleo Celular/fisiología , División del Núcleo Celular/fisiología , Células Cultivadas , Segregación Cromosómica/fisiología , Fase de Segmentación del Huevo/clasificación , Fase de Segmentación del Huevo/metabolismo , Fase de Segmentación del Huevo/fisiología , Formación de Concepto/fisiología , Femenino , Fertilización/genética , Humanos , Masculino , Oocitos/citología , Oocitos/metabolismo , Oocitos/fisiología , Proyectos Piloto , Estudios RetrospectivosRESUMEN
New Current Procedural Terminology (CPT) codes were adopted in 2004 for utilization with assisted reproductive technologies.
Asunto(s)
Técnicas de Laboratorio Clínico/normas , Registros Médicos/normas , Técnicas Reproductivas Asistidas , Fase de Segmentación del Huevo/clasificación , Criopreservación/métodos , Técnicas de Cultivo de Embriones , Transferencia de Embrión , Femenino , Líquido Folicular/fisiología , Control de Formularios y Registros , Humanos , Masculino , Oocitos/citología , Análisis de Semen/métodos , Recuperación de la Esperma , Conservación de Tejido/métodosRESUMEN
The policy of single embryo transfer (SET) adopted for women <36 years old since 1 July 2003, strongly calls for improvement of embryo selection. A total of 196 cycles in which SET was performed were randomly allocated to two groups. In the first group, early cleavage was assessed (ECA) 25 h after insemination. The embryo with the best score that cleaved early, if present, was selected for transfer. In the second group, early cleavage was not assessed (ECNA) and embryo selection was based solely on the embryo score. Ninety-eight cycles were allocated in the ECA and ECNA group respectively. Early cleavage occurred in 64% of cycles and 32.2% of embryos. Patient population and embryo morphology were similar between the two groups, and similar delivery rates were observed (27.6 versus 24.5% respectively in the ECA and ECNA groups). The assessment of early cleavage as additional parameter did not improve the delivery rate in the single embryo transfer policy.