RESUMEN
The infectious pancreatic necrosis virus (IPNV) is responsible for significant economic losses in the aquaculture industry. It is an unenveloped virus with an icosahedral capsid. Its viral genome comprises two dsRNA segments, A and B. Segment A contains a small ORF, which encodes VP5, and a large ORF, which encodes a polyprotein that generates the structural proteins and the viral protease. Segment B encodes the RNA-dependent RNA polymerase (RdRp), called VP1 in this free form, or Vpg when it covalently attaches to the viral RNA. The viral genome does not have cap or poly(A). Instead, each 5' end is linked to the Vpg. Recently, we demonstrated that mRNA-A contains an internal ribosome entry site (IRES) to command polyprotein synthesis. However, the presence of Vpg on IPNV mRNAs and its impact on cellular translation has not been investigated. This research demonstrates that IPNV mRNAs are linked to Vpg and that this protein inhibits cap-dependent translation on infected cells. Also, it is demonstrated that Vpg interacts with eIF4E and that rapamycin treatment partially diminishes the viral protein synthesis. In addition, we determined that an IRES does not command translation of IPNV mRNA-B. We show that VPg serves as a cap substitute during the initiation of IPNV translation, contributing to understanding the replicative cycle of Birnaviruses. Our results indicate that the viral protein VP1/Vpg is multifunctional, having a significant role during IPNV RNA synthesis as the RdRp and the primer for IPNV RNA synthesis and translation as the viral protein genome, acting as a cap substitute.
Asunto(s)
Virus de la Necrosis Pancreática Infecciosa , Virus de la Necrosis Pancreática Infecciosa/genética , Sitios Internos de Entrada al Ribosoma , Poliproteínas/genética , ARN Mensajero/genética , ARN Mensajero/metabolismo , ARN Viral/genética , ARN Viral/metabolismo , ARN Polimerasa Dependiente del ARN/genética , Proteínas Virales/genética , Proteínas Virales/metabolismoRESUMEN
Nannochloropsis oceanica is a unicellular oleaginous microalga of emerging biotechnological interest with a sequenced, annotated genome, available transcriptomic and proteomic data, and well-established basic molecular tools for genetic engineering. To establish N. oceanica as a eukaryotic host for recombinant protein synthesis and develop molecular technology for vaccine production, we chose the viral surface protein 2 (VP2) of a pathogenic fish virus that causes infectious pancreatic necrosis as a model vaccine. Upon stable nuclear transformation of N. oceanica strain CCMP1779 with the codon-optimized VP2 gene, a Venus reporter fusion served to evaluate the strength of different endogenous promoters in transformant populations by qPCR and flow cytometry. The highest VP2 yields were achieved for the elongation factor promoter, with enhancer effects by its N-terminal leader sequence. Individual transformants differed in their production capability of reporter-free VP2 by orders of magnitude. When subjecting the best candidates to kinetic analyses of growth and VP2 production in photobioreactors, recombinant protein integrity was maintained until the early stationary growth phase, and a high yield of 4.4% VP2 of total soluble protein was achieved. The maximum yield correlated with multiple integrations of the expression vector into the nuclear genome. The results demonstrate that N. oceanica was successfully engineered to constitute a robust platform for high-level production of a model subunit vaccine. The molecular methodology established here can likely be adapted in a straightforward manner to the production of further vaccines in the same host, allowing their distribution to fish, vertebrates, or humans via a microalgae-containing diet. KEY POINTS: ⢠We engineered N. oceanica for recombinant protein production. ⢠The antigenic surface protein 2 of IPN virus could indeed be expressed in the host. ⢠A high yield of 4.4% VP2 of total soluble protein was achieved in N. oceanica.
Asunto(s)
Virus de la Necrosis Pancreática Infecciosa , Estramenopilos , Vacunas Virales , Animales , Peces , Humanos , Virus de la Necrosis Pancreática Infecciosa/genética , Proteínas de la Membrana , Factores de Elongación de Péptidos , Proteómica , Proteínas Recombinantes/genética , Estramenopilos/genética , Vacunación , Vacunas Virales/genéticaRESUMEN
Infectious pancreatic necrosis (IPN) is a highly contagious disease of young salmonid fish and is one of the most severe economic diseases in aquaculture. In Turkey, an increase in infectious pancreatic necrosis virus (IPNV) outbreaks in freshwater rainbow trout have been reported in recent years. This study aimed to analyze the VP2 gene from recent IPNV isolates from Turkey to determine whether there are epidemiological links between IPNV isolates from rainbow trout (Oncorhynchus mykiss; 62) and sea bass (Dicentrarchus labrax; 1), wild turbot (Scophthalmus maximus; 1) and the environment in order to investigate potential wild and farmed fish interactions. In this study, 62 Turkish IPNV isolates collected over 10 years (2005-2014) from rainbow trout, sea bass and turbot were genotypically characterized. The phylogenetic analysis indicated that Turkish IPNV isolates are closely related to strains from Denmark, Iran and Spain and that all Turkish IPNV isolates belong to genogroup V, serotype A2 (Sp strain). Furthermore, low genetic diversity was found among the Turkish isolates (identity, 95.5%-100% nucleotides and 97.8%-100% amino acids). The result of the analysis of the amino acid residues found at positions 217, 221 and 247 (proline, threonine and alanine, respectively) could be associated with virulence.
Asunto(s)
Infecciones por Birnaviridae , Enfermedades de los Peces , Virus de la Necrosis Pancreática Infecciosa , Oncorhynchus mykiss , Animales , Infecciones por Birnaviridae/epidemiología , Infecciones por Birnaviridae/veterinaria , Enfermedades de los Peces/epidemiología , Virus de la Necrosis Pancreática Infecciosa/genética , Filogenia , Turquía/epidemiología , VirulenciaRESUMEN
Infectious pancreatic necrosis virus (IPNV) is the aetiological agent of a highly contagious disease that affects farmed salmonids. IPNV isolates have been phylogenetically classified into eight genogroups, of which two are present in Chile, genogroups 1 and 5. Here, we compare the mortality rate caused by isolates from both genogroups in rainbow trout (Oncorhynchus mykiss) fry to determine if there is an association between host susceptibility and phylogenetic characterization of IPNV. Fish were challenged by immersion with one of four isolates (two for each genogroup), and mortality curves were assessed after 30 days. Viral load was measured in all mortalities and in live fish sampled at 1, 7 and 20 days post-infection. Although mortality was low throughout the challenge, differences were found between fish infected with different isolates. Both isolates from genogroup 1 caused greater cumulative mortalities than either of the isolates from genogroup 5. When combined, the overall mortality rate of fish challenged with genogroup 1 isolates was significantly higher than those infected with genogroup 5. However, viral load was lower on trout infected with genogroup 1 isolates. These results suggest that rainbow trout are more susceptible to IPNV isolates from genogroup 1 than genogroup 5.
Asunto(s)
Infecciones por Birnaviridae/veterinaria , Enfermedades de los Peces/mortalidad , Virus de la Necrosis Pancreática Infecciosa/fisiología , Oncorhynchus mykiss , Carga Viral/veterinaria , Animales , Infecciones por Birnaviridae/mortalidad , Infecciones por Birnaviridae/virología , Chile/epidemiología , Enfermedades de los Peces/virología , Genotipo , Virus de la Necrosis Pancreática Infecciosa/genética , FilogeniaRESUMEN
Infectious hematopoietic necrosis virus (IHNV) and infectious pancreatic necrosis virus (IPNV) are important pathogens in rainbow trout farming worldwide. Their co-infection is also common, which causes great economic loss in juvenile salmon species. Development of a universal virus vaccine providing broadly cross-protective immunity will be of great importance. In this study, we generated two recombinant (r) virus (rIHNV-N438A-ΔNV-EGFP and rIHNV-N438A-ΔNV-VP2) replacing the NV gene of the backbone of rIHNV at the single point mutation at residue 438 with an efficient green fluorescent protein (EGFP) reporter gene and antigenic VP2 gene of IPNV. Meanwhile, we tested their efficacy against the wild-type (wt) IHNV HLJ-09 virus and IPNV serotype Sp virus challenge. The relative per cent survival rates of two recombinant viruses against (wt) IHNV HLJ-09 virus challenge were 84.6% and 81.5%, respectively. Simultaneously, the relative per cent survival rate of rIHNV-N438A-ΔNV-VP2 against IPNV serotype Sp virus challenge was 88.9%. It showed the two recombinant viruses had high protection rates and induced a high level of antibodies against IHNV or IPNV. Taken together, these results suggest the VP2 gene of IPNV can act as candidate gene for vaccine and attenuated multivalent live vaccines and molecular marker vaccines have potential application for viral vaccine.
Asunto(s)
Inmunidad Adaptativa , Enfermedades de los Peces/prevención & control , Virus de la Necrosis Hematopoyética Infecciosa/inmunología , Virus de la Necrosis Pancreática Infecciosa/inmunología , Oncorhynchus mykiss , Vacunas Virales/inmunología , Animales , Infecciones por Birnaviridae/inmunología , Infecciones por Birnaviridae/prevención & control , Infecciones por Birnaviridae/veterinaria , Enfermedades de los Peces/inmunología , Virus de la Necrosis Hematopoyética Infecciosa/genética , Virus de la Necrosis Pancreática Infecciosa/genética , Distribución Aleatoria , Infecciones por Rhabdoviridae/inmunología , Infecciones por Rhabdoviridae/prevención & control , Infecciones por Rhabdoviridae/veterinaria , Vacunas Sintéticas/administración & dosificación , Vacunas Sintéticas/genética , Vacunas Sintéticas/inmunología , Vacunas Virales/administración & dosificación , Vacunas Virales/genéticaRESUMEN
In order to obtain an insight into genomic changes and associated evolution and adaptation of Infectious Pancreatic Necrosis Virus (IPNV), the complete coding genomes of 57 IPNV isolates collected from Scottish aquafarms from 1982 to 2014 were sequenced and analysed. Phylogenetic analysis of the sequenced IPNV strains showed separate clustering of genogroups I, II, III and V. IPNV isolates with genetic reassortment of segment A/B of genogroup III/II were determined. About 59â% of the IPNV isolates belonged to the persistent type and 32â% to the low-virulent type, and only one highly pathogenic strain (1.79â%) was identified. Codon adaptation index calculations indicated that the IPNV major capsid protein VP2 has adapted to its salmonid host. Under-representation of CpG dinucleotides in the IPNV genome to minimize detection by the innate immunity receptors, and observed positive selection in the virulence determination sites of VP2 embedded in the variable region of the main antigenic region, suggest an immune escape mechanism driving virulence evolution. The prevalence of mostly persistent genotypes, together with the assumption of adaptation and immune escape, indicates that IPNV is evolving with the host.
Asunto(s)
Infecciones por Birnaviridae/veterinaria , Enfermedades de los Peces/epidemiología , Enfermedades de los Peces/virología , Variación Genética , Virus de la Necrosis Pancreática Infecciosa/clasificación , Virus de la Necrosis Pancreática Infecciosa/genética , Adaptación Biológica , Animales , Acuicultura , Infecciones por Birnaviridae/epidemiología , Infecciones por Birnaviridae/virología , Proteínas de la Cápside/genética , Codón , Genotipo , Evasión Inmune , Virus de la Necrosis Pancreática Infecciosa/aislamiento & purificación , Virus de la Necrosis Pancreática Infecciosa/patogenicidad , Epidemiología Molecular , Prevalencia , Escocia/epidemiología , Selección Genética , Análisis de Secuencia de ADN , Virulencia , Secuenciación Completa del GenomaRESUMEN
Infectious pancreatic necrosis virus (IPNV) is a common pathogen of rainbow trout (Oncorhynchus mykiss) in Turkey. We found that 455 of 1,676 sample pools tested were IPNV positive. Positive samples were found in all geographical regions where sampling was conducted. Sequence and phylogenetic analyses of VP2 from 30 isolates representing all regions showed that the viruses were highly similar in sequence and grouped within Genogroup 5 (serotype Sp-A2). No correlations between sequences, sampling sites or geographical origins were identified. Although clinical disease was evident in several farms, analyses of the amino acid sequence of VP2 showed that all virus strains harboured the P217 T221 motif, assumed to be associated with low virulence. We conclude that IPNV is prevalent in Turkish rainbow trout farms and that the viruses are very homogenous and likely to be of European origin. Frequent exchange of eggs and live fish within the farming industry may explain the homogeneity of the IPNV.
Asunto(s)
Infecciones por Birnaviridae/veterinaria , Enfermedades de los Peces/virología , Virus de la Necrosis Pancreática Infecciosa/aislamiento & purificación , Oncorhynchus mykiss/virología , Secuencias de Aminoácidos , Secuencia de Aminoácidos , Animales , Acuicultura , Infecciones por Birnaviridae/virología , Enfermedades de los Peces/epidemiología , Virus de la Necrosis Pancreática Infecciosa/genética , Filogenia , Serogrupo , Turquía/epidemiologíaRESUMEN
Infectious pancreatic necrosis (IPN) is an important restraint to production of salmonids in aquaculture globally. In order to implement efficacious mitigation strategies for control of this disease, it is important to understand infection routes under current production systems. IPN virus has been shown to be transmitted vertically in Rainbow trout, from broodstock to fingerlings in hatcheries, and there is circumstantial evidence suggesting that vertical transmission can also occur in Atlantic salmon, in addition to horizontal transmission between grow-out fish in farms. In this study, we show that the smolt carries infection with IPN from hatchery to the marine farm. We do this by comparing sequences from fish groups taken both in hatcheries and on corresponding marine grow-out farms. We use statistical analysis to prove that sequences obtained from the same fish group in both hatchery and marine farm are more similar than sequences obtained from random fish groups on hatcheries and marine farms.
Asunto(s)
Infecciones por Birnaviridae/veterinaria , Trazado de Contacto/métodos , Enfermedades de los Peces/transmisión , Virus de la Necrosis Pancreática Infecciosa/genética , Oncorhynchus mykiss/virología , Enfermedades Pancreáticas/veterinaria , Factores de Edad , Animales , Acuicultura , Infecciones por Birnaviridae/epidemiología , Infecciones por Birnaviridae/prevención & control , Infecciones por Birnaviridae/transmisión , Enfermedades de los Peces/epidemiología , Enfermedades de los Peces/virología , Explotaciones Pesqueras , Virus de la Necrosis Pancreática Infecciosa/aislamiento & purificación , Oncorhynchus mykiss/crecimiento & desarrollo , Oncorhynchus mykiss/fisiología , Enfermedades Pancreáticas/epidemiología , Enfermedades Pancreáticas/prevención & control , Enfermedades Pancreáticas/virología , Salmo salar/virología , Análisis de Secuencia de ADNRESUMEN
BACKGROUND: The infectious pancreatic necrosis virus (IPNV) causes significant economic losses in Chilean salmon farming. For effective sanitary management, the IPNV strains present in Chile need to be fully studied, characterized, and constantly updated at the molecular level. METHODS: In this study, 36 Chilean IPNV isolates collected over 6 years (2006-2011) from Salmo salar, Oncorhynchus mykiss, and Oncorhynchus kisutch were genotypically characterized. Salmonid samples were obtained from freshwater, estuary, and seawater sources from central, southern, and the extreme-south of Chile (35° to 53°S). RESULTS: Sequence analysis of the VP2 gene classified 10 IPNV isolates as genogroup 1 and 26 as genogroup 5. Analyses indicated a preferential, but not obligate, relationship between genogroup 5 isolates and S. salar infection. Fifteen genogroup 5 and nine genogroup 1 isolates presented VP2 gene residues associated with high virulence (i.e. Thr, Ala, and Thr at positions 217, 221, and 247, respectively). Four genogroup 5 isolates presented an oddly long VP5 deduced amino acid sequence (29.6 kDa). Analysis of the VP2 amino acid motifs associated with clinical and subclinical infections identified the clinical fingerprint in only genogroup 5 isolates; in contrast, the genogroup 1 isolates presented sequences predominantly associated with the subclinical fingerprint. Predictive analysis of VP5 showed an absence of transmembrane domains and plasma membrane tropism signals. WebLogo analysis of the VP5 BH domains revealed high identities with the marine birnavirus Y-6 and Japanese IPNV strain E1-S. Sequence analysis for putative 25 kDa proteins, coded by the ORF between VP2 and VP4, exhibited three putative nuclear localization sequences and signals of mitochondrial tropism in two isolates. CONCLUSIONS: This study provides important advances in updating the characterizations of IPNV strains present in Chile. The results from this study will help in identifying epidemiological links and generating specific biotechnological tools for controlling IPNV outbreaks in Chilean salmon farming.
Asunto(s)
Infecciones por Birnaviridae/veterinaria , Variación Genética , Virus de la Necrosis Pancreática Infecciosa/genética , Virus de la Necrosis Pancreática Infecciosa/aislamiento & purificación , Oncorhynchus kisutch/virología , Oncorhynchus mykiss/virología , Salmo salar/virología , Animales , Acuicultura , Infecciones por Birnaviridae/virología , Chile , Genotipo , Virus de la Necrosis Pancreática Infecciosa/clasificación , Análisis de Secuencia de ADN , Proteínas Estructurales Virales/genéticaRESUMEN
Viral hemorrhagic septicemia virus (VHSV) and infectious pancreatic necrosis virus (IPNV) are economically important pathogens of the salmonid aquaculture industry. In previous work we demonstrated that a cell line persistently infected with IPNV (EPCIPNV) exhibited antiviral activity against superinfection with the heterologous virus VHSV. This work extends our study by analyzing the replication of VHSV in the IPNV-persistently infected cells. At early and late stages of infection VHSV RNA synthesis, as well as VHSV-induced syncytia formation, were examined in EPCIPNV cultures. During the course of VHSV infection the accumulation of VHSV RNA is inhibited in EPCIPNV cells. Typical VHSV-induced membrane fusion at the late stages of infection is also absent in the IPNV carrier cultures. VHSV binding and fusion to EPCIPNV cells did not appear to be impaired, but a potent inhibitory effect on VHSV RNA synthesis is exerted at early times of infection in the IPNV carrier culture. In conclusion, the EPCIPNV cells are considered to be a useful system to study viral interference as well to analyze the mechanisms underlying the phenomenon of superinfection exclusion.
Asunto(s)
Enfermedades de los Peces/virología , Virus de la Necrosis Pancreática Infecciosa/fisiología , Novirhabdovirus/fisiología , Infecciones por Rhabdoviridae/veterinaria , Replicación Viral , Animales , Técnicas de Cultivo de Célula , Línea Celular , Virus de la Necrosis Pancreática Infecciosa/genética , Virus de la Necrosis Pancreática Infecciosa/crecimiento & desarrollo , Novirhabdovirus/genética , Novirhabdovirus/crecimiento & desarrollo , Infecciones por Rhabdoviridae/virología , Salmonidae/virología , Cultivo de VirusRESUMEN
Infectious pancreatic necrosis virus (IPNV) has been isolated annually since 1987 from salmonids without clinical signs at coastal fish farms in Finland. In the inland area, viral isolations were rare until 2012, when IPNV was detected at several freshwater fish farms. Between 2013 and 2015, the infection spread and IPNV was continuously isolated from several farms, both inland and on the coast. The aim of this study was to genetically characterise the IPNV isolates collected from Finnish coastal and inland fish farms over the last 15 years, and to detect genetic changes that may have occurred in the virus populations during the study period. The partial VP2 gene sequence from 88 isolates was analysed. In addition, a complete genomic coding sequence was obtained from 11 isolates. Based on the genetic analyses, Finnish IPNV isolates belong to three genogroups: 2, 5 and 6. The genetic properties of the isolates appear to vary between inland farms producing juveniles and food fish farms in the coastal region: the inland farms harboured genogroup 2 isolates, whereas at coastal farms, all three genogroups were detected. Little genetic variation was observed within the Finnish genogroup 2 and 5 isolates, whereas among the genogroup 6 isolates, two subgroups were detected. All isolates studied demonstrated amino acid patterns in the viral VP2 gene previously associated with avirulence. However, increased mortality was detected at some of the farms, indicating that more research is needed to clarify the relationship between the pathogenicity and genetic properties of IPNV isolates from different genogroups.
Asunto(s)
Infecciones por Birnaviridae/veterinaria , Enfermedades de los Peces/virología , Virus de la Necrosis Pancreática Infecciosa , Salmonidae , Animales , Acuicultura , Infecciones por Birnaviridae/epidemiología , Infecciones por Birnaviridae/virología , Finlandia/epidemiología , Regulación Viral de la Expresión Génica , Genoma Viral , Virus de la Necrosis Pancreática Infecciosa/genética , Filogenia , Proteínas Estructurales Virales/genética , Proteínas Estructurales Virales/metabolismoRESUMEN
An aquabirnavirus was isolated from diseased marbled eels (Anguilla marmorata; MEIPNV1310) with gill haemorrhages and associated mortality. Its genome segment sequences were obtained through next-generation sequencing and compared with published aquabirnavirus sequences. The results indicated that the genome sequence of MEIPNV1310 contains segment A (3099 nucleotides) and segment B (2789 nucleotides). Phylogenetic analysis showed that MEIPNV1310 is closely related to the infectious pancreatic necrosis Ab strain within genogroup II. This genome sequence is beneficial for studying the geographic distribution and evolution of aquabirnaviruses.
Asunto(s)
Aquabirnavirus/genética , Aquabirnavirus/aislamiento & purificación , Infecciones por Birnaviridae/veterinaria , Anguilas/virología , Enfermedades de los Peces/virología , Genoma Viral , Animales , Aquabirnavirus/clasificación , Aquabirnavirus/patogenicidad , Acuicultura , Infecciones por Birnaviridae/virología , Branquias/patología , Branquias/virología , Hemorragia/veterinaria , Hemorragia/virología , Secuenciación de Nucleótidos de Alto Rendimiento , Virus de la Necrosis Pancreática Infecciosa/genética , Filogenia , Análisis de Secuencia de ADN , TaiwánRESUMEN
Infectious pancreatic necrosis (IPN) is a significant disease of farmed salmonids resulting in direct economic losses due to high mortality in China. However, no gene sequence of any Chinese infectious pancreatic necrosis virus (IPNV) isolates was available. In the study, moribund rainbow trout fry samples were collected during an outbreak of IPN in Yunnan province of southwest China in 2013. An IPNV was isolated and tentatively named ChRtm213. We determined the full genome sequence of the IPNV ChRtm213 and compared it with previously identified IPNV sequences worldwide. The sequences of different structural and non-structural protein genes were compared to those of other aquatic birnaviruses sequenced to date. The results indicated that the complete genome sequence of ChRtm213 strain contains a segment A (3099 nucleotides) coding a polyprotein VP2-VP4-VP3, and a segment B (2789 nucleotides) coding a RNA-dependent RNA polymerase VP1. The phylogenetic analyses showed that ChRtm213 strain fell within genogroup 1, serotype A9 (Jasper), having similarities of 96.3% (segment A) and 97.3% (segment B) with the IPNV strain AM98 from Japan. The results suggest that the Chinese IPNV isolate has relative closer relationship with Japanese IPNV strains. The sequence of ChRtm213 was the first gene sequence of IPNV isolates in China. This study provided a robust reference for diagnosis and/or control of IPNV prevalent in China.
Asunto(s)
Enfermedades de los Peces/genética , Virus de la Necrosis Pancreática Infecciosa/genética , Oncorhynchus mykiss/virología , Secuencia de Aminoácidos/genética , Animales , Secuencia de Bases , China , Enfermedades de los Peces/virología , Virus de la Necrosis Pancreática Infecciosa/patogenicidad , Anotación de Secuencia Molecular , Oncorhynchus mykiss/genética , FilogeniaRESUMEN
Infectious pancreatic necrosis virus (IPNV) is an important virus which affects the salmonid aquaculture industry worldwide; therefore, it is important to develop rapid and reliable methods of diagnosis to detect the disease at early stages. Nowadays, RT-qPCR is replacing other methods because it provides additional information on the viral load, which is important to have a better understanding of the virus replication level and of the stage of the infection and its risk level. The main problem stems from the high diversity of this virus, which can compromise the reliability of the diagnosis. In this study, we have designed an RT-qPCR procedure for diagnosis and quantification of IPNV based on a single pair of primers targeted to segment B. The procedure has been validated, in vitro and in vivo, testing two different types of standards against seven reference strains and 23 field isolates from different types. The procedure is reliable for the detection of any type, with a detection limit of 31 TCID50 mL-1 , 50 pfu mL-1 or 66 RNA copies mL-1 , depending on the standard. All the standard curves showed high reliability (R2 > 0.95). The results support the high reliability of this new procedure for the diagnosis and quantification of IPNV.
Asunto(s)
Infecciones por Birnaviridae/veterinaria , Enfermedades de los Peces/diagnóstico , Virus de la Necrosis Pancreática Infecciosa/aislamiento & purificación , Perciformes , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/veterinaria , Salmón , Animales , Infecciones por Birnaviridae/diagnóstico , Infecciones por Birnaviridae/virología , Línea Celular , Cartilla de ADN/genética , Enfermedades de los Peces/virología , Tecnología Química Verde , Virus de la Necrosis Pancreática Infecciosa/clasificación , Virus de la Necrosis Pancreática Infecciosa/genética , Reproducibilidad de los Resultados , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/métodosRESUMEN
Infectious pancreatic necrosis (IPN) is a contagious viral disease of fish that causes economic losses in aquaculture worldwide. In Finland, IPN virus (IPNV) has been isolated since 1987 from adult fish showing no signs of clinical disease at fish farms located in the coastal areas of the Baltic Sea. The inland area of Finland, however, remained free of IPN until 2012, when fish on several rainbow trout farms were diagnosed IPNV-positive. The fish mortalities detected at the farms were low, but clinical signs and histopathological changes typical for IPNV infection were seen in juvenile salmonids. IPNV was isolated at high water temperatures up to 22°C. In 2013 and 2014, IPNV detections continued at inland farms, indicating that infections have spread. The aim of this study was to describe the epidemiology of the outbreak and to characterise the Finnish inland IPNV isolates using histopathological, immunohistochemical and genetic approaches. In order to determine the epidemiological origin of the inland IPNV infections, the partial viral capsid protein (VP2) gene sequences of the inland IPNV isolates were compared with the sequences of the isolates from the coastal farms. Based on the genetic analysis, the inland isolates belong to IPNV Genogroup 2 (Serotype A3/Ab), and the origin of the isolates appears to be one or several coastal fish farms.
Asunto(s)
Acuicultura , Infecciones por Birnaviridae/veterinaria , Enfermedades de los Peces/virología , Virus de la Necrosis Pancreática Infecciosa/patogenicidad , Oncorhynchus mykiss , Animales , Infecciones por Birnaviridae/epidemiología , Infecciones por Birnaviridae/virología , Finlandia/epidemiología , Enfermedades de los Peces/epidemiología , Virus de la Necrosis Pancreática Infecciosa/genética , FilogeniaRESUMEN
Leeches have been reported to harbor several important fish pathogens, including spring viremia of carp virus, infectious hematopoietic necrosis virus (IHNV), and viral hemorrhagic septicemia virus (VHSV), and also may contain blood protozoa. In the present study, leeches were collected from water bodies located in Kurdistan province, Iran. The specimens were tested for IHNV, VHSV, and infectious pancreatic necrosis virus (IPNV) using the PCR method. The results showed that two different species of leeches, Hemiclepsis marginata and Hirudo medicinalis, were infected by IPNV among the seven species studied. The infected leeches were found in areas that were polluted with untreated sewage coming from upstream fish farms culturing Rainbow Trout Oncorhynchus mykiss. In addition, the fish at fish farms in the vicinity had been infected with IPNV 9 months previously. Our results showed that the virus causing infectious pancreatic necrosis is present in the leeches H. marginata and H. medicinalis, suggesting that leeches are a potential source of IPNV in fish farms. Received October 14, 2015; accepted June 1, 2016.
Asunto(s)
Virus de la Necrosis Pancreática Infecciosa/aislamiento & purificación , Sanguijuelas/virología , Animales , Acuicultura , Hirudo medicinalis/virología , Virus de la Necrosis Hematopoyética Infecciosa/aislamiento & purificación , Virus de la Necrosis Pancreática Infecciosa/genética , Irán , Novirhabdovirus/genética , Novirhabdovirus/aislamiento & purificación , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/veterinaria , Especificidad de la EspecieRESUMEN
Infectious Pancreatic Necrosis Virus (IPNV) is a member of the family Birnaviridae which causes significant losses in the aquaculture industry. To develop a recombinant vaccine for IPNV, a cDNA construct of IPNV VP2-VP3 fusion gene was prepared and cloned into an Escherichia coli (E. coli) expression vector (pET-26b) to obtain recombinant protein products. A study was conducted to determine the antibody responses and protective capacity of this recombinant vaccine expressing VP2-VP3 fusion protein. Subsequently, juvenile rainbow trout were inoculated by injecting purified recombinant IPNV VP2-VP3 proteins, followed by challenge with virulent IPNV in rainbow trout. Our results demonstrate that recombinant E. coli derived VP2-VP3 fusion protein induced a strong and significantly (P < 0.05) higher IgM antibody response in serum samples compared to control groups. Following intraperitoneal challenge, the relative percent survival (RPS) rate of survivors was 83% for the vaccinated group. Statistical analysis of IgM levels indicated that immunogenicity of recombinant VP2-VP3 protein, combined with adjuvant, was much higher than any other groups of rainbow trout challenged with virulent IPNV. This result was confirmed by measuring the viral loads of IPNV in immunized rainbow trout which was drastically reduced, as analyzed by real-time RT-PCR. In summary, we demonstrate that E. coli-expressed IPNV VP2-VP3 injectable vaccine is highly immunogenic and protective against IPNV infection.
Asunto(s)
Infecciones por Birnaviridae/veterinaria , Enfermedades de los Peces/prevención & control , Virus de la Necrosis Pancreática Infecciosa/inmunología , Oncorhynchus mykiss , Vacunación/veterinaria , Proteínas Virales de Fusión/genética , Animales , Infecciones por Birnaviridae/inmunología , Infecciones por Birnaviridae/prevención & control , Infecciones por Birnaviridae/virología , Escherichia coli/genética , Enfermedades de los Peces/inmunología , Enfermedades de los Peces/virología , Virus de la Necrosis Pancreática Infecciosa/genética , Virus de la Necrosis Pancreática Infecciosa/metabolismo , Proteínas Recombinantes de Fusión/genética , Proteínas Recombinantes de Fusión/inmunología , Proteínas Virales de Fusión/inmunología , Carga Viral/veterinariaRESUMEN
Infectious pancreatic necrosis is a significant disease of farmed salmonids resulting in direct economic losses due to high mortality and disease-management costs. Significant outbreaks of the disease occurred in farmed Atlantic salmon in Ireland between 2003 and 2007, associated with imported ova and smolts. As the virus was known to occur in the country since the development of aquaculture in the 1980s, this study examined archived samples to determine whether these older isolates were associated with virulent forms. The study showed that two genotypes of IPNV were present in the 1990s, genotype 3 and genotype 5. A more virulent subtype of the virus first appeared in 2003 associated with clinical outbreaks of IPN, and this subtype is now the most prevalent form of IPNV found in the country. The data also indicated that IPNV in Ireland is more closely related to Scottish and continental European isolates than to Norwegian, Chilean and Australasian genogroup 5 isolates.
Asunto(s)
Infecciones por Birnaviridae/veterinaria , Brotes de Enfermedades , Enfermedades de los Peces/epidemiología , Virus de la Necrosis Pancreática Infecciosa/clasificación , Virus de la Necrosis Pancreática Infecciosa/genética , Filogenia , Salmo salar/virología , Animales , Acuicultura , Infecciones por Birnaviridae/epidemiología , Infecciones por Birnaviridae/virología , Análisis por Conglomerados , Enfermedades de los Peces/virología , Genotipo , Virus de la Necrosis Pancreática Infecciosa/aislamiento & purificación , Irlanda/epidemiología , Epidemiología Molecular , Datos de Secuencia Molecular , Análisis de Secuencia de ADN , Homología de SecuenciaRESUMEN
Infectious pancreatic necrosis virus (IPNV) is the etiological agent of a highly contagious disease that is endemic to salmon farming in Chile and causes great economic losses to the industry. Here we compared different diagnostic methods to detect IPNV in field samples, including 3 real-time reverse transcription PCR (qRT-PCR) assays, cell culture isolation, and indirect fluorescent antibody test (IFAT). Additionally, we performed a phylogenetic analysis to investigate the genogroups prevailing in Chile, as well as their geographic distribution and virulence. The 3 qRT-PCR assays used primers that targeted regions of the VP2 and VP1 genes of the virus and were tested in 46 samples, presenting a fair agreement within their results. All samples were positive for at least 2 of the qRT-PCR assays, 29 were positive for cell culture, and 23 for IFAT, showing less sensitivity for these latter 2 methods. For the phylogenetic analysis, portions of 1180 and 523 bp of the VP2 region of segment A were amplified by RT-PCR, sequenced and compared with sequences from reference strains and from isolates reported by previous studies carried out in Chile. Most of the sequenced isolates belonged to genogroup 5 (European origin), and 5 were classified within genogroup 1 (American origin). Chilean isolates formed clusters within each of the genogroups found, evidencing a clear differentiation from the reference strains. To our knowledge, this is the most extensive study completed for IPNV in Chile, covering isolates from sea- and freshwater salmon farms and showing a high prevalence of this virus in the country.
Asunto(s)
Infecciones por Birnaviridae/veterinaria , Enfermedades de los Peces/virología , Virus de la Necrosis Pancreática Infecciosa/genética , Oncorhynchus mykiss , Filogenia , Salmo salar , Animales , Infecciones por Birnaviridae/epidemiología , Infecciones por Birnaviridae/virología , Línea Celular , Chile/epidemiología , Enfermedades de los Peces/epidemiología , Técnica del Anticuerpo Fluorescente Indirecta , Regulación Viral de la Expresión Génica , Virus de la Necrosis Pancreática Infecciosa/aislamiento & purificación , Reacción en Cadena en Tiempo Real de la Polimerasa , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Proteínas Estructurales Virales/genética , Proteínas Estructurales Virales/metabolismoRESUMEN
Infectious pancreatic necrosis virus (IPNV) causes economic losses with a highly variable mortality rate worldwide, especially in rainbow trout. The virus has a double-stranded bi-partite RNA genome designated segment A and B. New complete genome sequences of nine rainbow trout isolates from Turkey were determined and subjected to phylogenetic analysis, identifying all as genotype 5 (serotype Sp). A time-dependent change in the extended pathogenicity motif of VP2 from P217T221A247 (PTA) to PTE P217T221E247 over a period of 10 years was identified. A wider analysis of 99 IPNV sequences from Turkey and Iran revealed the emergence of the motif PTE from 2007 to 2017, inducing significant morbidity in fry by 2013. In fact, displacement of the PTA motif, by the PTE motif in IPNV isolates appeared to be connected to a production peak of rainbow trout in 2013. An additional CAI analysis provided more evidence, indicating that rainbow trout culture in Turkey has an influence on the evolution of IPNV.