Evaluation of polymerase chain reaction for the detection of Paracoccidioides brasiliensis DNA on serum samples from patients with paracoccidioidomycosis
Mem. Inst. Oswaldo Cruz
; 101(2): 219-221, Mar. 2006.
Article
en En
| LILACS
| ID: lil-430900
Biblioteca responsable:
BR1.1
RESUMO
The aim of this study was to demonstrate the DNA of Paracoccidioides brasiliensis in human serum samples of patients with paracoccidioidomycosis using the polymerase chain reaction (PCR). The diagnosis of paracoccidioidomycosis (PCM) was defined by microscopic observation of the fungus on direct exam or histopathology, culture, and serological positivity. DNA from serum of 33 patients with PCM was extracted and submitted to nested-PCR using primers from the gp 43 gene. Only one sample was positive on nested-PCR. We conclude that the prevalence of fungemia in patients with different clinical forms of PCM is low, limiting the use of serum DNA detection as an alternative diagnostic tool.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
LILACS
Asunto principal:
Paracoccidioides
/
Paracoccidioidomicosis
/
ADN de Hongos
/
Proteínas Fúngicas
/
Glicoproteínas
/
Reacción en Cadena de la Polimerasa
/
Fungemia
/
Antígenos Fúngicos
Tipo de estudio:
Diagnostic_studies
/
Risk_factors_studies
Límite:
Adult
/
Child
/
Female
/
Humans
/
Male
País/Región como asunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
MEDICINA TROPICAL
/
PARASITOLOGIA
Año:
2006
Tipo del documento:
Article
País de afiliación:
Brasil