Cytotoxicity evaluation of multipurpose contact lens solutions using an in vitro test battery.
CLAO J
; 25(1): 28-35, 1999 Jan.
Article
en En
| MEDLINE
| ID: mdl-10073634
ABSTRACT
PURPOSE:
Many in vitro alternatives to eye irritation testing are not mechanism-specific and do not employ ocular cell lines. We have developed an effective and reliable test battery that reveals toxicity mechanisms of contact lens solutions on cell metabolism and proliferation.METHODS:
Cytotoxicity endpoints were quantified using bovine corneal epithelial cultures in 96-well microplates. A kenacid blue assay provided information on total cell protein, while lactate production and alamarBlue assays served as indicators of aerobic/anaerobic metabolism and redox state of cells grown in serum-free Dulbecco's modified Eagle's/Ham's F12 medium (DMEM/F12). Concentrations (% v/v) causing 10-90% inhibition of the control assay responses were used for correlations with in vivo data.RESULTS:
Cytotoxicities of contact lens solutions correlated better with irritant symptoms than with corneal staining, and were ranked as follows Lens Plus << Opti-Free < or = ContaClair < or = ReNu. Lens Plus was not toxic to cell glycolysis, respiration, and proliferation for up to 20% v/v. However, the multi-purpose solutions inhibited these endpoints in a concentration-dependent manner. Opti-Free and ReNu, containing Dymed and Polyquad (ammonium surfactants), showed non-specific cell inhibition. The lactate production assay had a flatter log concentration-response curve than the other two assays.CONCLUSIONS:
The proposed biochemically-based test battery using the target corneal epithelium has the potential to be a simple and effective method for screening and defining toxicity profiles of contact lens care solutions. The model can be applicable to small- or large-scale testing programs and research and development of new ocular products.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Oxazinas
/
Xantenos
/
Soluciones para Lentes de Contacto
/
Epitelio Corneal
Límite:
Animals
Idioma:
En
Revista:
CLAO J
Año:
1999
Tipo del documento:
Article
País de afiliación:
Australia