Expression of IgH chain genes in antibody response to ragweed and purified Amb a 1: evidence for differential regulation of isotype production.
Mol Immunol
; 36(9): 587-98, 1999 Jun.
Article
en En
| MEDLINE
| ID: mdl-10499812
ABSTRACT
A survey of the work with Ig response to allergens carried out previously reveals an allergen-specific response both by IgE and all of IgG subclasses. Response of non-sensitive people is characterized by the appearance of a variety of the IgG subclasses. We have reexamined ragweed and Amb a 1 specific Ig response in 54 nonsensitive and 147 atopic or atopic-allergic people using a new inverse sandwich immunoassay allowing discrimination based on antibody affinity. We show that non-sensitive people present no, 0 out of 54, Ig response with affinities higher than Ka 10(7) M(-1). The subpopulation of 66 atopics who never have experienced desensitization responds vigorously and solely (56 out of 66) with genes of the sequence gamma2-alpha2. Only ten showed an additional weak response from gamma1-alpha1. This suggests a possible association between the atopic state and selective activation of part of the gene sequence.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Proteínas de Plantas
/
Isotipos de Inmunoglobulinas
/
Alérgenos
/
Cadenas Pesadas de Inmunoglobulina
/
Hipersensibilidad
/
Hipersensibilidad Inmediata
Límite:
Humans
Idioma:
En
Revista:
Mol Immunol
Año:
1999
Tipo del documento:
Article
País de afiliación:
Estados Unidos