Microtubule growth activates Rac1 to promote lamellipodial protrusion in fibroblasts.
Nat Cell Biol
; 1(1): 45-50, 1999 May.
Article
en En
| MEDLINE
| ID: mdl-10559863
ABSTRACT
Microtubules are involved in actin-based protrusion at the leading-edge lamellipodia of migrating fibroblasts. Here we show that the growth of microtubules induced in fibroblasts by removal of the microtubule destabilizer nocodazole activates Rac1 GTPase, leading to the polymerization of actin in lamellipodial protrusions. Lamellipodial protrusions are also activated by the rapid growth of a disorganized array of very short microtubules induced by the microtubule-stabilizing drug taxol. Thus, neither microtubule shortening nor long-range microtubule-based intracellular transport is required for activating protrusion. We suggest that the growth phase of microtubule dynamic instability at leading-edge lamellipodia locally activates Rac1 to drive actin polymerization and lamellipodial protrusion required for cell migration.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Proteína de Unión al GTP rac1
/
Microtúbulos
Límite:
Animals
Idioma:
En
Revista:
Nat Cell Biol
Año:
1999
Tipo del documento:
Article
País de afiliación:
Estados Unidos