A panel of monoclonal antibodies to ovine placental lactogen.
J Endocrinol
; 165(2): 435-42, 2000 May.
Article
en En
| MEDLINE
| ID: mdl-10810307
ABSTRACT
A panel of 11 rat monoclonal antibodies (mAbs) has been raised to ovine placental lactogen (PL). By competitive enzyme-linked immunoabsorbent assay (ELISA), confirmed by two-site ELISA, the antibodies were shown to recognize six antigenic determinants on the ovine PL molecule, two of which overlap. One antigenic determinant (designated 1) was shared by other members of the prolactin/growth hormone (GH)/PL family in ruminants, humans and rodents. The binding of (125)I-labelled ovine PL to crude receptor preparations from sheep liver (somatotrophic) or rabbit mammary gland (lactogenic) was inhibited by mAbs recognizing antigenic determinants 2-6. Both types of receptor preparation were affected similarly. In the local in vivo pigeon crop sac assay, mAbs directed against determinants 3 and 6 enhanced the biological activity of ovine PL.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Lactógeno Placentario
/
Anticuerpos Monoclonales
/
Epítopos
Límite:
Animals
Idioma:
En
Revista:
J Endocrinol
Año:
2000
Tipo del documento:
Article
País de afiliación:
Reino Unido