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Development of a molecular probe to baboon interleukin-10 mRNA for in situ hybridization during experimental schistosomiasis.
Chou, T M; Stich, R W; Marsala, C; Scott, M; Brown, C C; Rawlings, C A; Damian, R T.
Afiliación
  • Chou TM; Department of Cellular Biology, University of Georgia, Athens, Georgia 30602, USA. wchou@fdah.com
Ann N Y Acad Sci ; 916: 410-6, 2000.
Article en En | MEDLINE | ID: mdl-11193655
A nucleic acid probe complementary to baboon interleukin 10 (IL-10) mRNA was developed for in situ hybridization. Highly conserved IL-10 protein sequences from several mammals were aligned to design oligonucleotide primers flanking a 270-bp sequence of the target cDNA. RNA was isolated from stimulated peripheral blood mononuclear cells (PBMC). IL-10 cDNA was reverse-transcribed from the total PBMC RNA and amplified with the polymerase chain reaction (PCR). Cloning and sequencing of the PCR product confirmed it to be of baboon IL-10 origin, with 97.8% identity to human and 100% identity to macaque mRNA sequences. The baboon IL-10 DNA probe hybridized in Southern blots to a 7.9-Kbp or 8.6-Kbp band after digestion of genomic baboon DNA with Bam H1 or Eco R1, respectively. Preliminary results with an antisense riboprobe derived from this sequence showed the presence of IL-10 mRNA in sections of granulomatous tissues.
Asunto(s)
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Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Esquistosomiasis / ARN Mensajero / Interleucina-10 / Enfermedades de los Primates Tipo de estudio: Diagnostic_studies Límite: Animals / Female / Humans Idioma: En Revista: Ann N Y Acad Sci Año: 2000 Tipo del documento: Article País de afiliación: Estados Unidos
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Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Esquistosomiasis / ARN Mensajero / Interleucina-10 / Enfermedades de los Primates Tipo de estudio: Diagnostic_studies Límite: Animals / Female / Humans Idioma: En Revista: Ann N Y Acad Sci Año: 2000 Tipo del documento: Article País de afiliación: Estados Unidos