A precise and efficient stereological method for determining murine lung metastasis volumes.
Am J Pathol
; 158(6): 1997-2003, 2001 Jun.
Article
en En
| MEDLINE
| ID: mdl-11395377
ABSTRACT
We have developed a computer-assisted stereological method based on unbiased principles for estimating metastasis volumes in mouse lungs. We evaluated this method using the transplantable Lewis lung carcinoma. Twenty-one days after subcutaneous inoculation of 10(6) Lewis lung cells into C57BL/6J mice, the mice had primary tumors with an average volume of 2300 mm(3). After perfusion fixation, the lungs were removed, embedded in OCT compound, snap-frozen, and processed for stereology. The metastasis volumes were estimated by application of the Cavalieri principle after evaluation of single sections from several evenly distributed tissue levels. The metastasis volume in a group of nine mice varied between 0.01 and 14.4 mm(3), with an average of 6.1 mm(3). The coefficient of variation was 0.9. The coefficient of error of the volume estimation was determined in five cases and varied from 0.08 to 0.23. Thus, the variation on the metastasis volumes that is achieved by this method contributes very little, 2.5%, to the total variance within the group of mice. In conclusion, we have developed an efficient and unbiased method to determine the metastasis burden in mouse lungs.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Procesamiento de Imagen Asistido por Computador
/
Carcinoma Pulmonar de Lewis
/
Metástasis de la Neoplasia
Tipo de estudio:
Evaluation_studies
Límite:
Animals
Idioma:
En
Revista:
Am J Pathol
Año:
2001
Tipo del documento:
Article
País de afiliación:
Dinamarca