ActA and human zyxin harbour Arp2/3-independent actin-polymerization activity.
Nat Cell Biol
; 3(8): 699-707, 2001 Aug.
Article
en En
| MEDLINE
| ID: mdl-11483954
ABSTRACT
The actin cytoskeleton is a dynamic network that is composed of a variety of F-actin structures. To understand how these structures are produced, we tested the capacity of proteins to direct actin polymerization in a bead assay in vitro and in a mitochondrial-targeting assay in cells. We found that human zyxin and the related protein ActA of Listeria monocytogenes can generate new actin structures in a vasodilator-stimulated phosphoprotein-dependent (VASP) manner, but independently of the Arp2/3 complex. These results are consistent with the concept that there are multiple actin-polymerization machines in cells. With these simple tests it is possible to probe the specific function of proteins or identify novel molecules that act upon cellular actin polymerization.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Polímeros
/
Proteínas Bacterianas
/
Citoesqueleto de Actina
/
Actinas
/
Proteínas del Citoesqueleto
/
Proteínas de la Membrana
/
Metaloproteínas
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Nat Cell Biol
Año:
2001
Tipo del documento:
Article
País de afiliación:
Francia