Difference in the way of macrophage recognition of target cells depending on their apoptotic states.
Cell Death Differ
; 8(11): 1113-22, 2001 Nov.
Article
en En
| MEDLINE
| ID: mdl-11687889
ABSTRACT
Dying cells are selectively eliminated from the organism by phagocytosis. Previous studies suggested the existence of some other phagocytosis marker(s) that function together with phosphatidylserine, the best-characterized phagocytosis marker. We obtained here a monoclonal antibody named PH2 that inhibited macrophage phagocytosis of late apoptotic or necrotic cells, but not of early apoptotic cells. On the other hand, phagocytosis of cells at any time during the process of apoptosis was inhibitable by phosphatidylserine-containing liposomes. Inhibition occurred even when target cells were preincubated with PH2 and separated from unbound antibodies. Moreover, PH2 bound to apoptotic cells at late stages more efficiently than to those at early stages, and it did not bind to normal cells unless their plasma membrane was permeabilized. These results suggest that the putative PH2 antigen is a novel phagocytosis marker that translocates to the cell surface at late stages of apoptosis, resulting in maximal recognition and engulfment by macrophages.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Fagocitosis
/
Apoptosis
/
Macrófagos
Límite:
Humans
Idioma:
En
Revista:
Cell Death Differ
Año:
2001
Tipo del documento:
Article
País de afiliación:
Japón