Overexpression of the nucleolar protein nucleophosmin/B23 in collagen lattice-cultured fibroblasts: potential role in the control of protein synthesis.
Mol Cell Biochem
; 229(1-2): 45-50, 2002 Jan.
Article
en En
| MEDLINE
| ID: mdl-11936846
ABSTRACT
Fibroblasts cultivated in tridimensional collagen lattices exhibit a downregulation of protein synthesis, related to decreased ribosomal RNA (rRNA) content and half life, when compared to monolayer cultivated cells. The involvement in this process of nucleophosmin/B23, a nucleolar phosphoprotein with ribonuclease properties, was checked. We compared production of nucleophosmin/B23 in monolayer and collagen lattice cultured fibroblasts. A significant increase of nucleophosmin/B23 mRNA levels was noticed in lattice-cultured fibroblasts vs monolayers (+154%, p < 0.05). A concomitant enhancement of nucleolar nucleophosmin/B23 content was found (+112%, p < 0.001). Simultaneously, ribonuclease activity contained in nucleolar extracts from collagen lattice-cultured fibroblasts was significantly increased (+54%, p < 0.01). These data demonstrate that extracellular collagen matrix induces the overexpression of nucleophosmin/B23, and suggest that the regulation of protein syntheses in collagen lattice cultures may be explained, at least partly, by an increased degradation of neosynthesized rRNAs dependent on nucleophosmin.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Biosíntesis de Proteínas
/
Proteínas Nucleares
/
Fibroblastos
Límite:
Adult
/
Humans
Idioma:
En
Revista:
Mol Cell Biochem
Año:
2002
Tipo del documento:
Article
País de afiliación:
Francia