The PtdIns3P phosphatase myotubularin is a cytoplasmic protein that also localizes to Rac1-inducible plasma membrane ruffles.
J Cell Sci
; 115(Pt 15): 3105-17, 2002 Aug 01.
Article
en En
| MEDLINE
| ID: mdl-12118066
ABSTRACT
Myotubularin, the phosphatase mutated in X-linked myotubular myopathy, was shown to dephosphorylate phosphatidylinositol 3-monophosphate (PtdIns3P) and was also reported to interact with nuclear transcriptional regulators from the trithorax family. We have characterized a panel of specific antibodies and investigated the subcellular localization of myotubularin. Myotubularin is not detected in the nucleus, and localizes mostly as a dense cytoplasmic network. Overexpression of myotubularin does not detectably affect vesicle trafficking in the mammalian cells investigated, in contrast to previous observations in yeast models. Both mutation of a key aspartate residue of myotubularin and dominant activation of Rac1 GTPase lead to the recruitment of myotubularin to specific plasma membrane domains. Localization to Rac1-induced ruffles is dependent on the presence of a domain highly conserved in the myotubularin family (that we named RID). We thus propose that myotubularin may dephosphorylate a subpool of PtdIns3P (or another related substrate) at the plasma membrane.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Proteínas Tirosina Fosfatasas
/
Fosfatos de Fosfatidilinositol
/
Miopatías Estructurales Congénitas
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Proteína de Unión al GTP rac1
/
Extensiones de la Superficie Celular
/
Citoplasma
/
Células Eucariotas
Límite:
Animals
/
Humans
Idioma:
En
Revista:
J Cell Sci
Año:
2002
Tipo del documento:
Article
País de afiliación:
Francia