Neutralising human recombinant antibodies to human cytomegalovirus glycoproteins gB and gH.
FEMS Immunol Med Microbiol
; 34(3): 237-44, 2002 Nov 15.
Article
en En
| MEDLINE
| ID: mdl-12423777
ABSTRACT
A phage antibody display library of single chain fragment variable (scFv) was applied to develop anti-HCMV glycoprotein B (gB) and glycoprotein H (gH) neutralising libraries. To enrich for specific scFvs, the phage antibody was panned against cytomegalovirus epitopes derived from the N-terminal part of gB, the C-terminal part of gB and the N-terminal part of gH (NETIYNTTLKYGDV, VTSGSTKD and AASEALDPHAFHLLLNTYGR). A number of clones were differentiated by Bst N1 fingerprinting. After isolation of specific clones against each peptide, the neutralising effect of each clone was assessed by plaque reduction assay. This resulted in the isolation of eight neutralising scFv antibodies with 51-63% neutralising effects. Sequence analysis of three neutralising clones revealed the amino acids specificity changes in heavy and light chains of antibody molecules.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Proteínas del Envoltorio Viral
/
Anticuerpos Antivirales
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
FEMS Immunol Med Microbiol
Asunto de la revista:
ALERGIA E IMUNOLOGIA
/
DOENCAS TRANSMISSIVEIS
/
MICROBIOLOGIA
Año:
2002
Tipo del documento:
Article
País de afiliación:
Reino Unido