Differential expression of a 70 kDa O-glycoprotein on T cells: a possible marker for naive and early activated murine T cells.
Cell Immunol
; 218(1-2): 34-45, 2002.
Article
en En
| MEDLINE
| ID: mdl-12470612
ABSTRACT
We purified a 70 kDa O-glycoprotein that binds to the GalNAc specific lectin from Amaranthus leucocarpus (ALLr) and determined its expression pattern on T lymphocytes from different murine lymphoid organs. High level of ALLr expression was demonstrated in 95-98% of both CD4(+)8(+) and CD4(-)8(+) thymocytes, and in 80-95% of CD8(+) T cells from peripheral blood, lymph nodes, and spleen, whereas a minor fraction of CD4(+)8(-) thymocytes (46-67%) and peripheral CD4(+) T cells (9-40%) showed low ALLr expression. Peripheral CD19(+) B cells were ALLr negative and most of the peripheral ALL(+) T cells showed a CD62L(hi)CD45RB(hi)CD44(lo/-) phenotype, indicating features of naive cells. Mitogenic activation of peripheral T cells increased 3-fold the number of ALL(+)CD4(+) T cells 24 h after stimulation, as opposed to a >80% decrease in CD8(+) T cells 72 h after stimulation. Our results suggest that ALL detects a non-described surface O-glycoprotein selectively expressed by naive CD8(+) T cells and by early activated CD4(+) T cells.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Glicoproteínas de Membrana
/
Glicoproteínas
/
Activación de Linfocitos
/
Receptores Mitogénicos
/
Subgrupos de Linfocitos T
/
Lectinas de Plantas
Límite:
Animals
Idioma:
En
Revista:
Cell Immunol
Año:
2002
Tipo del documento:
Article
País de afiliación:
México