Silencing of the gene coding for the epsilon subunit of DNA polymerase III slows down the growth rate of Escherichia coli populations.
FEBS Lett
; 546(2-3): 295-9, 2003 Jul 10.
Article
en En
| MEDLINE
| ID: mdl-12832057
ABSTRACT
Chromosome replication in Escherichia coli is accomplished by the multimeric enzyme DNA polymerase III; the relevance, in vivo, of the epsilon subunit (encoded by dnaQ) for processivity and fidelity of DNA polymerase III has been evaluated. To this aim, dnaQ has been conditionally silenced by means of in vivo expression of different antisense RNAs. Unexpectedly, the presence of the Shine-Dalgarno sequence is essential for the effectiveness of antisense constructs. Silencing of dnaQ induces a severe decrease in growth rate not paralleled by high mutation frequencies, suggesting that the epsilon subunit primarily affects the processivity of DNA polymerase III.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Silenciador del Gen
/
Proteínas de Escherichia coli
/
ADN Polimerasa III
/
Escherichia coli
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Exodesoxirribonucleasas
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Genes Bacterianos
Idioma:
En
Revista:
FEBS Lett
Año:
2003
Tipo del documento:
Article
País de afiliación:
Italia