Multiple group-specific sequencing primers for reliable and rapid DNA sequencing.
Mol Cell Probes
; 17(4): 203-10, 2003 Aug.
Article
en En
| MEDLINE
| ID: mdl-12944124
ABSTRACT
Pyrosequencing technology is a bioluminometric DNA sequencing method that employs a cascade of four enzymes to deliver sequence signals. To date this technology has been limited to the sequencing of short stretches of DNA. As an improvement to this technique, we have introduced a bacterial group-specific, multiple sequencing primer approach that circumvents sequencing of less informative semi-conservative regions of the 16S rRNA gene. This new approach is suitable for challenging templates, improving sequence data quality, avoiding sequencing of non-specific amplification products, lessening sequencing time, and moreover, this strategy should open the way for many new applications in the future. The group-specific, multiple sequencing primers can be applied in the Sanger dideoxy sequencing method as well. In addition, we have improved the chemistry of the Pyrosequencing system enabling sequencing of longer stretches of DNA, which allows numerous new applications.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Bacterias
/
ADN Bacteriano
/
ARN Ribosómico 16S
/
Reacción en Cadena de la Polimerasa
/
Análisis de Secuencia de ADN
/
Cartilla de ADN
/
Genes de ARNr
Idioma:
En
Revista:
Mol Cell Probes
Asunto de la revista:
BIOLOGIA MOLECULAR
/
BIOTECNOLOGIA
Año:
2003
Tipo del documento:
Article
País de afiliación:
Suecia