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Elevated cytosolic concentrations of SecA compensate for a protein translocation defect in Escherichia coli cells with reduced levels of negatively charged phospholipids.
Kusters, R; Huijbregts, R; de Kruijff, B.
Afiliación
  • Kusters R; Department of Biochemistry of Membranes, Centre for Biomembranes and Lipid Enzymology, University of Utrecht, The Netherlands.
FEBS Lett ; 308(1): 97-100, 1992 Aug 10.
Article en En | MEDLINE | ID: mdl-1379556
Cellular extracts from cells with reduced synthesis of negatively charged phospholipids were found to support in vitro translocation of the precursor of the outer membrane protein PhoE with increased efficiency. Analysis of these extracts revealed that they contain increased levels of SecA. SecA depletion resulted in a loss of the translocation stimulatory activity, which could be restored by re-addition of purified SecA. We conclude that elevated cytosolic levels of SecA counteract the reduction of translocation efficiency due to low levels of negatively charged phospholipids in the inner membrane.
Asunto(s)
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Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteínas de Transporte de Membrana / Fosfolípidos / Proteínas Bacterianas / Adenosina Trifosfatasas / Proteínas de Escherichia coli / Citosol / Escherichia coli Idioma: En Revista: FEBS Lett Año: 1992 Tipo del documento: Article País de afiliación: Países Bajos
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Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteínas de Transporte de Membrana / Fosfolípidos / Proteínas Bacterianas / Adenosina Trifosfatasas / Proteínas de Escherichia coli / Citosol / Escherichia coli Idioma: En Revista: FEBS Lett Año: 1992 Tipo del documento: Article País de afiliación: Países Bajos