Determination of the catecholamines and serotonin, their precursors tyrosine and tryptophan, and their main metabolites in rat brain using reversed-phase high-performance liquid chromatography with fluorimetric and oxidative amperometric detection in series.
J Chromatogr
; 578(1): 17-30, 1992 Jul 01.
Article
en En
| MEDLINE
| ID: mdl-1400783
A high-performance liquid chromatographic method for the determination of catecholamines and serotonin, their precursors and their main metabolites was developed applied to rat cerebellum, hypothalamus, striatum and cortex. A fluorimetric and an oxidative amperometric detector were used in series. For both detectors, detection limits (25-520 pg) were useful for this application, linearity of standards was excellent (average r greater than 0.9997), between-run precision for sample analytes was generally acceptable (coefficient of variation less than 10% with appreciable concentrations present) and average recoveries of standard additions to sample analytes were better than 90%. Particular attention was paid to peak identification, including both a thorough treatment of retention time agreement of peaks in standards and sample analytes, and a comparison of results for the seven compounds amenable to quantitation by both detectors. Considerable attention was also given to determining the stability of standards and sample analytes under a wide variety of conditions, and practical recommendations were made.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Tirosina
/
Triptófano
/
Química Encefálica
/
Catecolaminas
/
Serotonina
/
Cromatografía Líquida de Alta Presión
Tipo de estudio:
Diagnostic_studies
/
Guideline
Límite:
Animals
Idioma:
En
Revista:
J Chromatogr
Año:
1992
Tipo del documento:
Article
País de afiliación:
Australia