Identification of a polymorphic Plasmodium vivax microsatellite marker.
Am J Trop Med Hyg
; 69(4): 377-9, 2003 Oct.
Article
en En
| MEDLINE
| ID: mdl-14640496
ABSTRACT
Microsatellite markers derived from simple sequence repeats have been useful in studying a number of human pathogens, including the human malaria parasite Plasmodium falciparum. Genetic markers for P. vivax would likewise help elucidate the genetics and population characteristics of this other important human malaria parasite. We have identified a locus in a P. vivax telomeric clone that contains simple sequence repeats. Primers were designed to amplify this region using a two-step semi-nested polymerase chain reaction protocol. The primers did not amplify template obtained from non-infected individuals, nor DNA from primates infected with the other human malaria parasites (P. ovale, P. malariae, or P. falciparum). The marker was polymorphic in P. vivax-infected field isolates obtained from Papua New Guinea, Indonesia and Guyana. This microsatellite marker may be useful in genetic and epidemiologic studies of P. vivax malaria.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Plasmodium vivax
/
Reacción en Cadena de la Polimerasa
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ADN Protozoario
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Malaria Vivax
/
Repeticiones de Microsatélite
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
/
Prognostic_studies
Límite:
Adult
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Animals
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Child
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Humans
Idioma:
En
Revista:
Am J Trop Med Hyg
Año:
2003
Tipo del documento:
Article
País de afiliación:
Estados Unidos