Evaluation of a convenient enzyme immunoassay to assess the quality of genital specimens submitted for the detection of human papillomavirus DNA by consensus PCR.
J Clin Virol
; 29(2): 127-33, 2004 Feb.
Article
en En
| MEDLINE
| ID: mdl-14747032
ABSTRACT
BACKGROUND:
In the PGMY-line blot assay, a human beta-globin fragment is co-amplified with human papillomavirus (HPV) DNA, and both analytes are detected by hybridization with probes fixed on a strip in a linear array. The beta-globin DIG-MWP test also detects beta-globin amplicons, but in a microtiter plate-based enzyme immunoassay format. Although the PGMY-line blot assay detected 50 cells per test, the beta-globin DIG-MWP test generated a signal above the detection cut-off with five cells per test.OBJECTIVE:
The performance of the beta-globin DIG-MWP assay to detect beta-globin DNA was assessed. STUDYDESIGN:
The beta-globin DIG-MWP assay was compared to a standard beta-globin PCR and to the PGMY-line blot strips on 401 genital specimens. Overall, the three beta-globin assays were compared on 325 undiluted lysates, 14 diluted lysates and DNA extracted from 62 lysate samples.RESULTS:
Concordance between the PGMY-line blot and the standard beta-globin assay reached 99.5% (399 of 401 results), for a kappa value of 0.95. Concordant results were also obtained between the beta-globin DIG-MWP assay and PGMY-line blot assay for 387 (96.5%) of 401 test results, for a kappa value of 0.57. Discordant results were due to the increased sensitivity of the DIG-MWP assay. Using a cut-off for positivity at 1.500 optical density (OD) units for beta-globin DIG-MWP, concordance improved to 100% (401 of 401 results, kappa at 1.00).CONCLUSION:
The beta-globin DIG-MWP assay was adequate to screen for sample adequacy for HPV analysis in genital specimens.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Papillomaviridae
/
ADN
/
ADN Viral
/
Globinas
/
Cuello del Útero
/
Técnicas para Inmunoenzimas
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
Límite:
Female
/
Humans
Idioma:
En
Revista:
J Clin Virol
Asunto de la revista:
VIROLOGIA
Año:
2004
Tipo del documento:
Article
País de afiliación:
Canadá