Cyclic enterobacterial common antigen: potential contaminant of bacterially expressed protein preparations.
J Biomol NMR
; 29(2): 199-204, 2004 Jun.
Article
en En
| MEDLINE
| ID: mdl-15014233
ABSTRACT
We have previously reported the identification of the cyclic enterobacterial common antigen (ECA(CYC)) polysaccharide in E. coli strains commonly used for heterologous protein expression (PJA Erbel et al., J. Bacteriol. 185 (2003) 1995). Following this initial report, interactions among several NMR groups established that characteristic N -acetyl signals of ECA(CYC) have been observed in (15)N-(1)H HSQC spectra of samples of various bacterially-expressed proteins suggesting that this water-soluble carbohydrate is a common contaminant. We provide NMR spectroscopic tools to recognize ECA(CYC) in protein samples, as well as several methods to remove this contaminant. Early recognition of ECA-based NMR signals will prevent time-consuming analyses of this copurifying carbohydrate.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Polisacáridos Bacterianos
/
Espectroscopía de Resonancia Magnética
/
Antígenos Bacterianos
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
J Biomol NMR
Asunto de la revista:
BIOLOGIA MOLECULAR
/
DIAGNOSTICO POR IMAGEM
/
MEDICINA NUCLEAR
Año:
2004
Tipo del documento:
Article
País de afiliación:
Estados Unidos