Definitive hematopoiesis requires the mixed-lineage leukemia gene.
Dev Cell
; 6(3): 437-43, 2004 Mar.
Article
en En
| MEDLINE
| ID: mdl-15030765
ABSTRACT
The Mixed-Lineage Leukemia (MLL) gene encodes a Trithorax-related chromatin-modifying protooncogene that positively regulates Hox genes. In addition to their well-characterized roles in axial patterning, Trithorax and Polycomb family proteins perform less-understood functions in vertebrate hematopoiesis. To define the role of MLL in the development of the hematopoietic system, we examined the potential of cells lacking MLL. Mll-deficient cells could not develop into lymphocytes in adult RAG-2 chimeric animals. Similarly, in vitro differentiation of B cells required MLL. In chimeric embryos, Mll-deficient cells failed to contribute to fetal liver hematopoietic stem cell/progenitor populations. Moreover, we show that aorta-gonad-mesonephros (AGM) cells from Mll-deficient embryos lacked hematopoietic stem cell (HSC) activity despite their ability to generate hematopoietic progeny in vitro. These results demonstrate an intrinsic requirement for MLL in definitive hematopoiesis, where it is essential for the generation of HSCs in the embryo.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Células Madre
/
Proto-Oncogenes
/
Regulación del Desarrollo de la Expresión Génica
/
Proteínas de Unión al ADN
/
Hematopoyesis
Idioma:
En
Revista:
Dev Cell
Asunto de la revista:
EMBRIOLOGIA
Año:
2004
Tipo del documento:
Article