Post-transcriptional regulation of the cystic fibrosis gene in cardiac development and hypertrophy.
Biochem Biophys Res Commun
; 319(2): 410-8, 2004 Jun 25.
Article
en En
| MEDLINE
| ID: mdl-15178422
ABSTRACT
Eukaryotic gene expression, reflected in the amount of steady-state mRNA, is regulated at the post-transcriptional level. The 5'-untranslated regions (5'-UTRs) of some transcripts contain cis-acting elements, including upstream open reading frames (uORFs), that have been identified as being fundamental in modulating translation efficiency and mRNA stability. Previously, we demonstrated that uORFs present in the 5'-UTR of cystic fibrosis transmembrane conductance regular (CFTR) transcripts expressed in the heart were able to modulate translation efficiency of the main CFTR ORF. Here, we show that the same 5'-UTR elements are associated with the differential stability of the 5'-UTR compared to the main coding region of CFTR transcripts. Furthermore, these post-transcriptional mechanisms are important factors governing regulated CFTR expression in the heart, in response to developmental and pathophysiological stimuli.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Procesamiento Postranscripcional del ARN
/
Cardiomegalia
/
Regulación del Desarrollo de la Expresión Génica
/
Regulador de Conductancia de Transmembrana de Fibrosis Quística
/
Corazón
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
2004
Tipo del documento:
Article
País de afiliación:
Australia