[HUP98-HOXA9 transgenic mice are susceptible to N-ethyl-N-nitrosourea stimulation in leukemogenesis].
Zhonghua Xue Ye Xue Za Zhi
; 25(5): 257-61, 2004 May.
Article
en Zh
| MEDLINE
| ID: mdl-15182530
ABSTRACT
OBJECTIVE:
In order to investigate the leukemogenic potential of NUP98-HOXA9 fusion gene in vivo.METHODS:
Molecular cloning technology was used to construct NUP98-HOXA9 transgenic plasmid and NUP98-HOXA9 transgenic mice were generated. The genotype and phenotype of the NUP98-HOXA9 transgenic mice were analyzed by PCR, RT-PCR and colony-forming assay. The effect of N-ethyl-N-nitrosourea (ENU) stimulation on the transgenic mice was analyzed by peripheral blood count, bone marrow (BM) cells morphology pathological examination.RESULTS:
The transgenic expression was detected in 5 independent lines of NUP98-HOXA9 transgenic mice, but no expected phenotypes was found in 2 year follow-up. Upon ENU stimulation, 2 of 10 transgenic mice developed myeloid leukemia, suggesting that NUP98-HOXA9 transgenic mice have increased susceptibility to ENU mutagenesis in leukemogenesis.CONCLUSION:
The fusion gene expressed in BM cells of NUP98-HOXA9 transgenic mice. It seems that the expression of the fusion gene is insufficient to trigger leukemogenesis. However, the increased susceptibility to ENU mutagenesis suggests that NUP98-HOXA9 fusion gene might play a potential role in leukemogenesis.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Leucemia Mieloide
/
Proteínas de Fusión Oncogénica
/
Proteínas de Homeodominio
/
Proteínas de Complejo Poro Nuclear
Límite:
Animals
/
Female
/
Humans
/
Male
Idioma:
Zh
Revista:
Zhonghua Xue Ye Xue Za Zhi
Año:
2004
Tipo del documento:
Article
País de afiliación:
China