[Expression of proteinase cathepsin L1 gene of Schistosoma japonicum in Escherichia coli].
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi
; 22(2): 76-9, 2004 Apr 30.
Article
en Zh
| MEDLINE
| ID: mdl-15281444
ABSTRACT
OBJECTIVE:
To express the proteinase cathepsin L1 gene of Schistosoma japonicum (SjCL1) in Escherichia coli JM109 cells.METHODS:
The SjCL1 gene was amplified from the recombinant plasmid pcDNA3-SjCL1 by PCR. The gene was cloned into a prokaryotic expression vector pGEX4T-1 to construct a recombinant plasmid pGEX-SjCL1. The E. coli JM109 cells were transformed with the recombinant plasmid pGEX-SjCL1 and the transformants were induced by IPTG to express the recombinant protein, the target protein was then identified by SDS-PAGE and Western blotting.RESULTS:
A 1 kb length PCR product was obtained and a recombinant plasmid pGEX-SjCL1 was constructed. The expression product was detected by SDS-PAGE and Western blotting and an expression band about 62000 was found.CONCLUSION:
The SjCL1 gene is effectively expressed in the E. coli JM109 cells.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Schistosoma japonicum
/
Proteínas Recombinantes
/
Catepsinas
Límite:
Animals
Idioma:
Zh
Revista:
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi
Asunto de la revista:
PARASITOLOGIA
Año:
2004
Tipo del documento:
Article
País de afiliación:
China