Characterization of the BUD31 gene of Saccharomyces cerevisiae.
Biochem Biophys Res Commun
; 320(4): 1342-50, 2004 Aug 06.
Article
en En
| MEDLINE
| ID: mdl-15303280
ABSTRACT
A number of genes have been identified in the fully sequenced genome of Saccharomyces cerevisiae that appear to be conserved throughout evolution and the function of which remains poorly understood. In this manuscript we describe the initial characterization of yeast BUD31 gene. cDNA sequences highly related to BUD31 have been identified in human, Xenopus laevis, and Caenorhabditis elegans. With the aim of further understanding its function, we generated a BUD31-null yeast strain and characterized its phenotype bud31l mutant cells showed severe cytoskeletal abnormalities, with dramatic effects on actin distribution and bud formation. We also proceeded to identify interacting proteins using the tandem affinity-purification method, coupled to mass spectrometry Bud3lp was found in complex with proteins involved in mRNA splicing. We propose that the observed phenotypes for bud31-null strain could be the result of defective splicing and indicate a first functional role for Bud3lp and its homologs.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Saccharomyces cerevisiae
/
Empalme del ARN
/
Proteínas de Saccharomyces cerevisiae
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
2004
Tipo del documento:
Article
País de afiliación:
Italia