Ultrastructure of in situ perfusion-fixed avian liver, with special reference to structure of the sinusoids.
Microsc Res Tech
; 65(1-2): 101-11, 2004 Sep.
Article
en En
| MEDLINE
| ID: mdl-15570590
ABSTRACT
Broiler chicken and laying hen livers were fixed using a simple technique of in situ puncture perfusion of cacodylate-buffered fixative, which allowed characterisation of the fine structure of hepatic parenchyma, hepatocytes, bile ductules, and, in particular, the sinusoidal cells including endothelial, Kupffer, and Ito cells. Sinusoidal endothelial cells with their bulging perinuclear cytoplasm, evident in both transmission and scanning electron micrographs, were easily distinguishable from Kupffer cells, which possessed numerous pseudopodia. Bile ductular epithelium and hepatocytes of the laying hens contained large amounts of lipid. The ultrastructural characteristics of intercalated cells (putative extra-sinusoidal macrophages of chicken liver) are described and their possible role as precursors of Kupffer cells is discussed.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Fijación del Tejido
/
Hígado
/
Macrófagos
Límite:
Animals
Idioma:
En
Revista:
Microsc Res Tech
Asunto de la revista:
DIAGNOSTICO POR IMAGEM
Año:
2004
Tipo del documento:
Article