[Effects of Epimedium pubescens icariine on proliferation and differentiation of human osteoblasts].
Zhongguo Zhong Yao Za Zhi
; 30(4): 289-91, 2005 Feb.
Article
en Zh
| MEDLINE
| ID: mdl-15724410
ABSTRACT
OBJECTIVE:
To study the effects of epimedium pubescens icariine on the proliferation and differentiation of human osteoblasts.METHOD:
Human osteoblasts were obtained by inducting human marrow mesenchymal stem cells (hMSCs) directionally. MTT was used to observe the proliferation and activity of ALP was assayed to observe the differentiation of the third passage human osteoblasts cultured in vitro. The expression of BMP-2 mRNA was checked by RT-PCR.RESULT:
Epimedium pubescens icariine at the dose of 20 microg x mL(-1) increased greatly the proliferation and differentiation of human osteoblasts and promoted the expression of BMP-2 mRNA.CONCLUSION:
Epimedium pubescens icariine enhances significantly the proliferation and differentiation of human osteoblasts, which may be mediated by increasing the expression of BMP-2 mRNA.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Osteoblastos
/
Flavonoides
/
Factor de Crecimiento Transformador beta
/
Proteínas Morfogenéticas Óseas
/
Epimedium
Límite:
Humans
Idioma:
Zh
Revista:
Zhongguo Zhong Yao Za Zhi
Asunto de la revista:
FARMACOLOGIA
/
TERAPIAS COMPLEMENTARES
Año:
2005
Tipo del documento:
Article
País de afiliación:
China