Production of eosinophil chemoattractant activity by ovine gastrointestinal nematodes.
Vet Immunol Immunopathol
; 107(1-2): 57-65, 2005 Aug 15.
Article
en En
| MEDLINE
| ID: mdl-15982478
ABSTRACT
Eosinophilia is a well documented feature of helminth infections but the precise nature of the interaction between parasite and eosinophil remains an enigma. This paper describes experiments demonstrating that ruminant gastrointestinal trichostrongyles produce potent chemoattractant activity for ovine bone marrow-derived eosinophils in vitro. This activity was initially identified as a constituent of whole worm extracts of third and fourth larval (L3, L4), and adult stages of Teladorsagia circumcincta, and adult Haemonchus contortus. Similar activity was detected in excretory/secretory (E/S) material derived from live T. circumcincta L3. Subsequently, by adapting the assay technique to incorporate live worms directly into the system, it was shown that L3 of both T. circumcincta and H. contortus produced eosinophil chemoattractant activity. In contrast, neither whole worm extracts, or E/S preparations from mixed stages of the free-living nematode Caenorhabditis elegans contained eosinophil chemoattractant activity, and there was no evidence of chemoattractant production by live C. elegans. The results described are challenging to the traditional dogma that eosinophils are host-protective effector cells, and raise the intriguing possibility that ovine nematodes actively encourage recruitment of eosinophils. Local eosinophil-mediated mucosal damage, comparable to that seen in the asthmatic lung, may then provide a permissive local microenvironment for the parasite. Moreover, if they prove important for pathogenicity, nematode chemoattractants could offer future potential as novel therapeutic targets.
Buscar en Google
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Trichostrongyloidea
/
Ovinos
/
Factores Quimiotácticos Eosinófilos
/
Tracto Gastrointestinal
/
Haemonchus
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Vet Immunol Immunopathol
Año:
2005
Tipo del documento:
Article
País de afiliación:
Reino Unido