Infectious bursal disease virus (IBDV) induces apoptosis in chicken B cells.
Comp Immunol Microbiol Infect Dis
; 28(4): 321-37, 2005 Jul.
Article
en En
| MEDLINE
| ID: mdl-16188316
The ability of infectious bursal disease virus (IBDV) serotypes 1 and 2, and the role of VP4 of both serotypes as well as the capacity of three IBDV intermediate serotype 1-specific vaccine strains to induce apoptosis in a chicken B-lymphocyte cell line, DT40, were investigated using the TUNEL technique. It was observed that IBDV serotype 1 infected the DT40 cell line and directly induced apoptosis. In contrast, the non-pathogenic serotype 2 neither infected nor induced apoptosis, but was able to reduce the serotype 1-induced apoptosis when the two viruses were present in combination. VP4 of both serotypes did not induce apoptosis. IBDV VP2 of serotype 2 induced apoptosis in the same proportion and intensity as VP2 of serotype 1. IBDV intermediate vaccines varied in their ability to induce apoptosis in the DT40 cell line, which was also decreased-delayed in presence of serotype 2 IBDV. We hypothesize that both serotypes compete for the same receptor in DT-40 cells, and suggest that IBDV-induced apoptosis is a multistep process involving virus replication, protein expression, and release of virions.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Enfermedades de las Aves de Corral
/
Linfocitos B
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Pollos
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Apoptosis
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Infecciones por Birnaviridae
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Virus de la Enfermedad Infecciosa de la Bolsa
Límite:
Animals
Idioma:
En
Revista:
Comp Immunol Microbiol Infect Dis
Año:
2005
Tipo del documento:
Article