Stabilization of white blood cells and immunologic markers for extended analysis using flow cytometry.
J Immunol Methods
; 305(2): 107-19, 2005 Oct 30.
Article
en En
| MEDLINE
| ID: mdl-16214501
ABSTRACT
We evaluated whole blood samples drawn from 25 healthy donors and 20 HIV-infected donors into K3EDTA and Cyto-Chex BCT blood collection tubes for CD4, CD8, and CD3 cell counts (HIV Panel). Samples collected in Cyto-Chex BCT were stored at room temperature and tested by 4-color flow cytometry at 6 h, 3 days, and 7 days after isolation for CD4, CD8, and CD3 absolute cell counts/microl and compared with samples collected in K3EDTA tubes and tested at 6 h. Regardless of donor type, the samples collected in Cyto-Chex BCT and tested on day 7 yielded results that were statistically indistinguishable (with correlation coefficients of 0.96 or greater) from samples collected in K3EDTA tubes and tested at 6 h. We conclude that whole blood samples collected in Cyto-Chex BCT are stabilized for their marker phenotype for at least 7 days after phlebotomy.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Conservación de la Sangre
/
Leucocitos Mononucleares
/
Infecciones por VIH
/
Citometría de Flujo
Límite:
Adult
/
Female
/
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
J Immunol Methods
Año:
2005
Tipo del documento:
Article
País de afiliación:
Estados Unidos