Rapid PCR confirmation of E. coli O157:H7 after evanescent wave fiber optic biosensor detection.
Biosens Bioelectron
; 21(6): 881-7, 2005 Dec 15.
Article
en En
| MEDLINE
| ID: mdl-16257656
ABSTRACT
Escherichia coli O157H7 is an enteric pathogen of public health importance, which is monitored by several government agencies. Many rapid detection tests have been developed to identify foodstuff and water supplies contaminated by E. coli O157H7. However, these methods can be time consuming (24-48 h) due to the need to culture the bacteria to confirm detection results. Fiber optic biosensors can rapidly detect pathogens from complex matrices, yet confirmation tests can take up to 10h to complete. In addition, fiber optic biosensors can also be used to reduce the impact of PCR inhibitors present in complex matrices such as food and water. This paper presents methodologies to reduce the time necessary for confirmation from 10 to about 2 h, by direct PCR of bacteria from the fiber optic waveguides without the need for culture or enrichment steps.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Técnicas Biosensibles
/
Recuento de Colonia Microbiana
/
Reacción en Cadena de la Polimerasa
/
Escherichia coli O157
/
Tecnología de Fibra Óptica
/
Anticuerpos Antibacterianos
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
/
Prognostic_studies
Idioma:
En
Revista:
Biosens Bioelectron
Asunto de la revista:
BIOTECNOLOGIA
Año:
2005
Tipo del documento:
Article
País de afiliación:
Estados Unidos