Induction of thromboxane synthase and prostaglandin endoperoxide synthase mRNAs in human erythroleukemia cells by phorbol ester.
FEBS Lett
; 306(2-3): 161-4, 1992 Jul 20.
Article
en En
| MEDLINE
| ID: mdl-1633871
ABSTRACT
The effects of 12-O-tetradecanoyl-phorbol-13-acetate (TPA) on the mRNA levels of two enzymes, thromboxane synthase (TXS) and prostaglandin endoperoxide synthase (PES), responsible for the synthesis of thromboxane A2 from arachidonic acid, were studied in human erythroleukemia (HEL) cells by RNA blot analysis. TPA induced both TXS and PES mRNAs in HEL cells in a dose-dependent manner at 36 h. The half-maximal and maximal effects for the induction of both mRNAs were at approximately 3 x 10(-9) M and at 10(-8) M, respectively. TXS and PES mRNA levels increased in a time-dependent fashion by TPA, and reached to 7- and 3.5-fold of the control, respectively after 48 h of TPA treatment. These results suggest that expression of TXS and PES genes in HEL cells were simultaneously stimulated by TPA.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Tromboxano-A Sintasa
/
Acetato de Tetradecanoilforbol
/
Inhibidores de la Ciclooxigenasa
Límite:
Humans
Idioma:
En
Revista:
FEBS Lett
Año:
1992
Tipo del documento:
Article
País de afiliación:
Japón