A novel experimental approach for systematic identification of box H/ACA snoRNAs from eukaryotes.
Nucleic Acids Res
; 33(22): e194, 2005 Dec 15.
Article
en En
| MEDLINE
| ID: mdl-16361266
ABSTRACT
Box H/ACA snoRNAs represent an abundant group of small non-coding RNAs mainly involved in the pseudouridylation of rRNAs and/or snRNAs in eukaryotes and Archaea. In this study, we describe a novel experimental method for systematic identification of box H/ACA snoRNAs from eukaryotes. In the specialized cDNA libraries constructed by this method with total cellular RNAs from human blood cells, the high efficiency of cloning for diverse box H/ACA snoRNAs was achieved and seven novel species of this snoRNA family were identified from human for the first time. Furthermore, the novel method has been successfully applied for the identification of the box H/ACA snoRNAs from Drosophila and the fission yeast, demonstrating a powerful ability for systematic analysis of box H/ACA snoRNAs in a broad spectrum of eukaryotes.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Biblioteca de Genes
/
ARN Nucleolar Pequeño
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Nucleic Acids Res
Año:
2005
Tipo del documento:
Article