Mitochondrial location of severe acute respiratory syndrome coronavirus 3b protein.
Mol Cells
; 21(2): 186-91, 2006 Apr 30.
Article
en En
| MEDLINE
| ID: mdl-16682811
ABSTRACT
Severe acute respiratory syndrome-associated coronavirus (SARS-CoV), a distant member of the Group 2 coronaviruses, has recently been identified as the etiological agent of severe acute respiratory syndrome (SARS). The genome of SARS-CoV contains four structural genes that are homologous to genes found in other coronaviruses, as well as six subgroup-specific open reading frames (ORFs). ORF3 encodes a predicted 154-amino-acid protein that lacks similarity to any known protein, and is designated 3b in this article. We reported previously that SARS-CoV 3b is predominantly localized in the nucleolus, and induces G0/G1 arrest and apoptosis in transfected cells. In this study, we show that SARS-CoV 3b fused with EGFP at its N- or C- terminus co-localized with a mitochondria-specific marker in some transfected cells. Mutation analysis of SARS-CoV 3b revealed that the domain spanning amino acids 80 to 138 was essential for its mitochondria localization. These results provide new directions for studies of the role of SARS-CoV 3b protein in SARS pathogenesis.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Proteínas no Estructurales Virales
/
Coronavirus Relacionado al Síndrome Respiratorio Agudo Severo
/
Mitocondrias
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Mol Cells
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2006
Tipo del documento:
Article
País de afiliación:
China