[Spectral analysis and study of the microsome cytochrome P450 aromatase of Thais clavigera].

ABSTRACT

The microsomes were prepared from the viscera masses of Thais clavigeras. It is well known that there are some main components in microsome cytochrome P450 aroamtase, including cytochrome P450, cytochrome b5 and NADPH-cytochrome P450 reductase. So the contents of cytochrome P450 and cytochrome b5, and the activity of NADPH-cytochrome P450 reductase were determined by Shimadzu UV Visible spectrophotometer. And the microsomal proteins were analysed by SDS-PAGE. All the results indicated that there exists P450 arom in the T. clavigeras.