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An effective cold inducible expression system developed in Pseudoalteromonas haloplanktis TAC125.
Papa, Rosanna; Rippa, Valentina; Sannia, Giovanni; Marino, Gennaro; Duilio, Angela.
Afiliación
  • Papa R; Department of Organic Chemistry and Biochemistry, Federico II University of Naples, Napoli, Italy.
J Biotechnol ; 127(2): 199-210, 2007 Jan 01.
Article en En | MEDLINE | ID: mdl-16959351
A regulative two-component system previously identified in Pseudoalteromonas haloplanktis TAC125 was used to construct a cold inducible expression system that is under the control of l-malate. Performances of the inducible system were tested for both psychrophilic and mesophilic protein production using two "difficult" proteins as control. The results obtained demonstrated that both psychrophilic beta-galactosidase and yeast alpha-glucosidase are produced in a fully soluble and catalytically competent form. Optimal conditions for protein production, including growth temperature, growth medium and l-malate concentration were also investigated. Under optimized conditions yields of 620 and 27 mg/l were obtained for beta-galactosidase and alpha-glucosidase, respectively.
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Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteínas Recombinantes / Regulación Bacteriana de la Expresión Génica / Frío / Pseudoalteromonas Idioma: En Revista: J Biotechnol Asunto de la revista: BIOTECNOLOGIA Año: 2007 Tipo del documento: Article País de afiliación: Italia
Buscar en Google
Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteínas Recombinantes / Regulación Bacteriana de la Expresión Génica / Frío / Pseudoalteromonas Idioma: En Revista: J Biotechnol Asunto de la revista: BIOTECNOLOGIA Año: 2007 Tipo del documento: Article País de afiliación: Italia