Expression of foreign epitopes in P-fimbriae of Escherichia coli.
Mol Gen Genet
; 222(2-3): 297-303, 1990 Jul.
Article
en En
| MEDLINE
| ID: mdl-1703267
ABSTRACT
Hypervariable regions (HRs) of the major subunit of F11 fimbriae were exploited for insertion of foreign epitopes. Two insertion vectors were created that contain a unique cloning site in HR1 or HR4 respectively. Several oligonucleotides, coding for antigenic determinants derived from different pathogens, were cloned in both insertion vectors. Hybrid fimbrial subunits were generally shown to be assembled in fimbriae when the length of the inserted peptide did not exceed 14 amino acids. The inserted peptides appeared to be exposed in the fimbrial filament. One hybrid fimbrial protein induced detectable levels of antibodies against the inserted epitope if injected into mice.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Fimbrias Bacterianas
/
Escherichia coli
/
Epítopos
/
Antígenos Bacterianos
Límite:
Animals
Idioma:
En
Revista:
Mol Gen Genet
Año:
1990
Tipo del documento:
Article
País de afiliación:
Países Bajos