Arginine, citrulline and nitrate concentrations in the cerebrospinal fluid from patients with acute hydrocephalus.
J Chromatogr B Analyt Technol Biomed Life Sci
; 851(1-2): 250-6, 2007 May 15.
Article
en En
| MEDLINE
| ID: mdl-17110176
ABSTRACT
Citrulline and nitric oxide (NO) are synthesized by NO synthase (NOS) in a 11-stoichiometry. In this study, we determined by HPLC arginine and citrulline concentrations by fluorescence detection and nitrate levels by UV absorbance detection in the cerebrospinal fluid (CSF) from patients with acute hydrocephalus that underwent ventricular drainage. We found increased citrulline concentration (50.6+/-17.2 versus 20.9+/-2.0 microM) and decreased arginine/citrulline molar ratio (0.42+/-0.11 versus 1.12+/-0.16) in hydrocephalus patients, while arginine and nitrate concentrations and citrulline/nitrate molar ratio remained with little change. Citrulline has been determined as a marker of NOS activity in some studies, but it remains to be determined the extent at which this statement holds true, since other biochemical pathways also regulate the concentration of this amino acid. Our results suggest that citrulline is primarily synthesized from NOS in acute hydrocephalus. The evaluation of sample deproteinization by addition of methanol for the analysis of amino acids in CSF is also reported.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Arginina
/
Citrulina
/
Hidrocefalia
/
Nitratos
Tipo de estudio:
Observational_studies
Límite:
Adult
/
Humans
/
Middle aged
Idioma:
En
Revista:
J Chromatogr B Analyt Technol Biomed Life Sci
Asunto de la revista:
ENGENHARIA BIOMEDICA
Año:
2007
Tipo del documento:
Article
País de afiliación:
México