[Evaluation of estrogenic activity for nonylphenol using quantitative real-time RT-PCR method].
Huan Jing Ke Xue
; 27(9): 1825-8, 2006 Sep.
Article
en Zh
| MEDLINE
| ID: mdl-17117640
ABSTRACT
It has been demonstrated that nonylphenol (NP) exerts estrogenic activity. Quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) was used to study the VTG-I , VTG-II , CHG-H and CHG-L genes expression in the liver of juvenile medaka exposed to NP at 1, 10, 50, 100 microg/I. for 60 days. The results show that the VTG-I , VTG-II, CHG-H and CHG-L genes expression in the liver of juvenile medaka are induced even at 1 microg/L, significantly. It should be noted that the lowest-observed-effect concentration (LOECs) based on the hepatic vitellogenin (VTG) induction is about 1 microg/L, suggesting that quantitative real-time RT-PCR can detect the estrogenic activity of NP at relatively low concentration, and there is a potential application in evaluating the estrogenic activity of NP in aquatic environment.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Fenoles
/
Oryzias
/
Vitelogeninas
Límite:
Animals
Idioma:
Zh
Revista:
Huan Jing Ke Xue
Año:
2006
Tipo del documento:
Article
País de afiliación:
China