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[Evaluation of estrogenic activity for nonylphenol using quantitative real-time RT-PCR method].
Sai, Lin-lin; Zhang, Zhao-bin; Hu, Jian-ying; Hou, Yan-feng; Sai, Dao-jian.
Afiliación
  • Sai LL; College of Environmental Science, Peking University, Beijing 100871, China. sailinlin2009@163.com
Huan Jing Ke Xue ; 27(9): 1825-8, 2006 Sep.
Article en Zh | MEDLINE | ID: mdl-17117640
ABSTRACT
It has been demonstrated that nonylphenol (NP) exerts estrogenic activity. Quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) was used to study the VTG-I , VTG-II , CHG-H and CHG-L genes expression in the liver of juvenile medaka exposed to NP at 1, 10, 50, 100 microg/I. for 60 days. The results show that the VTG-I , VTG-II, CHG-H and CHG-L genes expression in the liver of juvenile medaka are induced even at 1 microg/L, significantly. It should be noted that the lowest-observed-effect concentration (LOECs) based on the hepatic vitellogenin (VTG) induction is about 1 microg/L, suggesting that quantitative real-time RT-PCR can detect the estrogenic activity of NP at relatively low concentration, and there is a potential application in evaluating the estrogenic activity of NP in aquatic environment.
Asunto(s)
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Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Fenoles / Oryzias / Vitelogeninas Límite: Animals Idioma: Zh Revista: Huan Jing Ke Xue Año: 2006 Tipo del documento: Article País de afiliación: China
Buscar en Google
Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Fenoles / Oryzias / Vitelogeninas Límite: Animals Idioma: Zh Revista: Huan Jing Ke Xue Año: 2006 Tipo del documento: Article País de afiliación: China