Nuclear respiratory factor 2 activates transcription of human mitochondrial translation initiation factor 2 gene.
Mitochondrion
; 7(3): 195-203, 2007 May.
Article
en En
| MEDLINE
| ID: mdl-17161026
ABSTRACT
We studied the transcriptional regulation of the human mitochondrial translation initiation factor 2 (IF2mt) gene. The minimal promoter region for the human IF2mt gene contains binding sites for Nuclear Respiratory Factor 2 (NRF-2), which is often involved in the transcription of mitochondrial-related genes. Electrophoresis mobility shift assay (EMSA) analyses indicated that NRF-2alpha/beta binds to the IF2mt promoter. Reporter assays, where HEK293T cells were co-transfected with an NRF-2alpha/beta-expressing vector and/or an IF2mt promoter reporter vector, revealed that NRF-2 trans-activates the IF2mt promoter. NRF-2 sites were also found in the promoters of several other mitochondrial translation factors, which suggests NRF-2 may play a key role in the regulation of mitochondrial protein synthesis.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Transcripción Genética
/
Regulación de la Expresión Génica
/
Factor 2 Procariótico de Iniciación
/
Factor de Transcripción de la Proteína de Unión a GA
Límite:
Humans
Idioma:
En
Revista:
Mitochondrion
Año:
2007
Tipo del documento:
Article
País de afiliación:
Japón