[Effects of antisense RNA of connective tissue growth factor expressing plasmid on rat liver fibrosis].
Zhonghua Gan Zang Bing Za Zhi
; 15(2): 118-21, 2007 Feb.
Article
en Zh
| MEDLINE
| ID: mdl-17362637
ABSTRACT
OBJECTIVE:
To observe the effects of antisense RNA of connective tissue growth factor (CTGF) on rat liver fibrosis.METHODS:
Gene recombinant techniques were used to construct a rat antisense RNA of CTGF recombinant plasmid which could be expressed in eukaryotic cells. The recombinant plasmids were encapsulated with lipofectamine and then transducted into a carbon tetrachloride (CCl4) induced rat liver fibrosis model. Expression of CTGF was assessed by RT-PCR, Western blot and immunohistochemistry. Immunohistochemistry was used to identify type I and III collagens. HE stained liver slides were used for pathological study.RESULTS:
The mRNA and protein expression of CTGF in the fibrotic liver transfected with antisense-CTGF were significantly decreased compared with those of the controls (P<0.01). The depositions of type I and type III collagens were also decreased (P<0.05). Antisense-CTGF also minimized the pathological fibrosis in the rat livers (P<0.01).CONCLUSION:
The results demonstrate that the antisense RNA of CTGF recombinant plasmid has certain effects in preventing liver fibrosis and makes it a possible candidate for use in future gene therapy.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
ARN Mensajero
/
ARN sin Sentido
/
Factor de Crecimiento del Tejido Conjuntivo
/
Cirrosis Hepática Experimental
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
Zh
Revista:
Zhonghua Gan Zang Bing Za Zhi
Asunto de la revista:
GASTROENTEROLOGIA
Año:
2007
Tipo del documento:
Article
País de afiliación:
China