Antimitotic agents increase the production of doubled-haploid embryos from cork oak anther culture.
J Plant Physiol
; 164(12): 1595-604, 2007 Dec.
Article
en En
| MEDLINE
| ID: mdl-17485138
ABSTRACT
The objective of this study is to induce the nuclear DNA duplication of anther-derived embryos of cork oak (Quercus suber L.) to obtain doubled-haploid plants. Anther culture of this species produces a low percentage (7.78%) of spontaneous diploids, as assessed by flow cytometry. Therefore, three antimitotic agents, colchicine, oryzalin and amiprophos-methyl (APM), were applied in vitro to anther-derived cork oak haploid embryos from six genotypes at different concentrations and for different treatment durations. Antimitotic toxicity was determined by embryo survival. Efficiency in inducing chromosome doubling of haploid embryos was evaluated by flow cytometry measurements and differences were observed between treatments. Nuclear DNA duplication and embryo survival of cork oak haploid embryos was most efficiently induced with oryzalin 0.01 mM for 48 h. Around 50% diploid embryos were obtained. The rate of chromosome duplication induced by APM 0.01 mM was also acceptable but lower than that induced by oryzalin, regardless of the duration of the treatment. Colchicine 1.3 or 8.8 mM was the least efficient, with the induction of necrosis and only a small rate of nuclear DNA duplication.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Semillas
/
Quercus
/
Flores
/
Antimitóticos
/
Haploidia
Idioma:
En
Revista:
J Plant Physiol
Asunto de la revista:
BOTANICA
Año:
2007
Tipo del documento:
Article
País de afiliación:
España