Requirements for PCNA monoubiquitination in human cell-free extracts.
DNA Repair (Amst)
; 6(12): 1726-31, 2007 Dec 01.
Article
en En
| MEDLINE
| ID: mdl-17669698
The Rad6/Rad18-dependent monoubiquitination of PCNA plays a crucial role in regulating replication past DNA damage in eukaryotic cells. We show here that in human cell-free extracts, efficient PCNA monoubiquitination requires both the synthesis of relatively long DNA tracts and polymerase idling or stalling at sites of DNA modification or DNA secondary structures. This dual dependency suggests a dynamic process in which, following initiation, the DNA synthesizing complex undergoes modifications that make it competent as a mediator for the activation of the Rad6/Rad18 pathway.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Antígeno Nuclear de Célula en Proliferación
/
Ubiquitina
Límite:
Humans
Idioma:
En
Revista:
DNA Repair (Amst)
Asunto de la revista:
BIOLOGIA MOLECULAR
/
BIOQUIMICA
Año:
2007
Tipo del documento:
Article
País de afiliación:
Francia