Comparative genomic hybridization detects secondary chromosomal deletions in Escherichia coli K-12 MG1655 mutants and highlights instability in the flhDC region.
J Bacteriol
; 189(24): 8786-92, 2007 Dec.
Article
en En
| MEDLINE
| ID: mdl-17921306
ABSTRACT
The use of whole-genome microarrays for monitoring mutagenized or otherwise engineered genetic derivatives is a potentially powerful tool for checking genomic integrity. Using comparative genomic hybridization of a number of unrelated, directed deletion mutants in Escherichia coli K-12 MG1655, we identified unintended secondary genomic deletions in the flhDC region in delta fnr, delta crp, and delta creB mutants. These deletions were confirmed by PCR and phenotypic tests. Our findings show that nonmotile progeny are found in some MG1655 directed deletion mutants, and studies on the effects of gene knockouts should be viewed with caution when the mutants have not been screened for the presence of secondary deletions or confirmed by other methods.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Transactivadores
/
Eliminación de Secuencia
/
Proteínas de Escherichia coli
/
Inestabilidad Genómica
/
Proteínas de Unión al ADN
/
Escherichia coli
/
Hibridación de Ácido Nucleico
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
J Bacteriol
Año:
2007
Tipo del documento:
Article
País de afiliación:
Reino Unido