Stimulation by glutamine and proline of HGF production in hepatic stellate cells.
Biochem Biophys Res Commun
; 363(4): 978-82, 2007 Nov 30.
Article
en En
| MEDLINE
| ID: mdl-17923118
ABSTRACT
Amino acids regulate cellular functions in a variety of cell types. Most notably, leucine stimulates protein production through the mammalian target of rapamycin (mTOR)-dependent signaling pathway. We investigated the effect of amino acids on hepatocyte growth factor (HGF) production. Treatment with glutamine and proline, as well as leucine, increased HGF levels in the culture medium of a rat hepatic stellate cell clone in a dose-dependent manner. Up-regulation of phosphorylation of 70 kDa ribosomal protein S6 kinase and eukaryotic initiation factor 4E-binding protein 1 was not apparent in the cells after treatment with glutamine or proline. When rats received injections of glutamine or proline, hepatic and circulating HGF levels increased and peaked around 12h after treatment. Glutamine and proline may have the potential to stimulate HGF production but the mechanism underlying this stimulation seems not to be through the mTOR-dependent signaling pathway.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Prolina
/
Factor de Crecimiento de Hepatocito
/
Hepatocitos
/
Glutamina
Límite:
Animals
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
2007
Tipo del documento:
Article
País de afiliación:
Japón