Inhibition of tetrandrine on epidermal growth factor-induced cell transformation and its signal transduction.
Anticancer Res
; 27(5A): 3187-93, 2007.
Article
en En
| MEDLINE
| ID: mdl-17970060
ABSTRACT
BACKGROUND:
The mouse epidermal JB6 cell system is a model for studying tumor promotion. We used the JB6 Cl 41 cell line to examine the mechanism of the anti-tumor-promoting effect of tetrandrine, an alkaloid isolated from Stephania tetrandra S Moore. MATERIALS ANDMETHODS:
The anti-tumor-promoting effect of tetrandrine was evaluated by assay of inhibition of epidermal growth factor (EGF)-induced transformation of JB6 Cl 41 cells in soft agar. The activity of activator protein-1 (AP-1), a transcription factor, was analyzed using the AP-1-dependent reporter assay. Phosphorylation of extracellular-signal regulated kinases (ERKs) and Akt, a pivotal effector of phosphatidylinositol 3-kinase (P13K), was detected by Western blotting.RESULTS:
Tetrandrine significantly blocked EGF-induced cell transformation, attenuated EGF-induced AP-1 activation, and inhibited phosphorylation of ERKs, which regulates AP-1 activation. It also tended to suppress EGF-induced Akt phosphorylation.CONCLUSION:
Our results indicate that tetrandrine inhibits EGF-induced transformation of JB6 cells by blocking the activation of ERKs, AP-1 and Akt.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Transformación Celular Neoplásica
/
Bencilisoquinolinas
/
Alcaloides
/
Factor de Crecimiento Epidérmico
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Anticancer Res
Año:
2007
Tipo del documento:
Article
País de afiliación:
Japón