Yeast cell-surface expression of chitosanase from Paenibacillus fukuinensis.
Biosci Biotechnol Biochem
; 71(11): 2845-7, 2007 Nov.
Article
en En
| MEDLINE
| ID: mdl-17986777
ABSTRACT
To produce chitoorigosaccharides using chitosan, we attempted to construct Paenibacillus fukuinensis chitosanase-displaying yeast cells as a whole-cell biocatalyst through yeast cell-surface engineering. The localization of the chitosanase on the yeast cell surface was confirmed by immunofluorescence labeling of cells. The chitosanase activity of the constructed yeast was investigated by halo assay and the dinitrosalicylic acid method.
Buscar en Google
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Saccharomyces cerevisiae
/
Bacillus
/
Proteínas Bacterianas
/
Biotecnología
/
Proteínas Recombinantes
/
Membrana Celular
/
Glicósido Hidrolasas
Idioma:
En
Revista:
Biosci Biotechnol Biochem
Asunto de la revista:
BIOQUIMICA
/
BIOTECNOLOGIA
Año:
2007
Tipo del documento:
Article
País de afiliación:
Japón